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Lentil (Lens culinaris Medik.) is an excellent source of protein and carbohydrates and is also rich in essential trace elements for the human diet. Selenium (Se) is an essential micronutrient for human health and nutrition, providing protection against several diseases and regulating important biological systems. Dietary intake of 55 μg of Se per day is recommended for adults, with inadequate Se intake causing significant health problems. The objective of this study was to identify and map quantitative trait loci (QTL) of genes controlling Se accumulation in lentil seeds using a population of 96 recombinant inbred lines (RILs) developed from the cross “PI 320937” × “Eston” grown in three different environments for two years (2012 and 2013). Se concentration in seed varied between 119 and 883 μg/kg. A linkage map consisting of 1,784 markers (4 SSRs, and 1,780 SNPs) was developed. The map spanned a total length of 4,060.6 cM, consisting of 7 linkage groups (LGs) with an average distance of 2.3 cM between adjacent markers. Four QTL regions and 36 putative QTL markers, with LOD scores ranging from 3.00 to 4.97, distributed across two linkage groups (LG2 and LG5) were associated with seed Se concentration, explaining 6.3–16.9% of the phenotypic variation.

IntroductionLentil, Lens culinaris Medic., is an important cool season food legume in the old world. It is a self-pollinated, diploid (2n = 2x = 14) with a genome size of 4063 Mbp (Arumuganathan and Earl, 1991). The origin of cultivated lentil is the

This study evaluated Mn concentration in the seeds of 120 RILs of lentil developed from the cross “CDC Redberry” × “ILL7502”. Micronutrient analysis using atomic absorption spectrometry indicated mean seed manganese (Mn) concentrations ranging from 8.5 to 26.8 mg/kg, based on replicated field trials grown at three locations in Turkey in 2012 and 2013. A linkage map of lentil was constructed and consisted of seven linkage groups with 5,385 DNA markers. The total map length was 973.1 cM, with an average distance between markers of 0.18 cM. A total of 6 QTL for Mn concentration were identified using composite interval mapping (CIM). All QTL were statistically significant and explained 15.3–24.1% of the phenotypic variation, with LOD scores ranging from 3.00 to 4.42. The high-density genetic map reported in this study will increase fundamental knowledge of the genome structure of lentil, and will be the basis for the development of micronutrient-enriched lentil genotypes to support biofortification efforts.

Soil chemical and microbial parameters are commonly used as soil quality indicators to evaluate sustainable land management in agroecosystems. The objective of this research was to evaluate the impact of organic and conventional management strategies on biological, chemical and yield parameters in vineyards (Vitis vinifera cv. Sultani seedless). Organic plots received a mixture of barley, vetch and broad bean (25 þ 35 þ 75 kg seed ha 21) as green manure, and farmyard manure (15 t ha 21 ) every year. Inorganic fertilizers (NPK) and pesticides were used in the conventional managed vineyards. Conventional and mulch tillage methods were used in conventional and organic plots, respectively. Microbiological and chemical soil properties were determined in the soil samples which were taken four times in 2004, 2005, 2006 and 2007. In addition, the yield and some quality parameters of vineyards were also determined. Soil microbial biomass, and dehydrogenase, b-glucosidase and alkaline phosphatase activity were significantly higher in organic management than in conventional management. Higher C mic /C org and lower qCO 2 values were found with the organic management. The response of the chemical indicators (C org , N t and available nutrient content) of the soils to different management systems appeared after a longer time than with the biological indicators. In the transition zone between continental and Mediterranean climates of West Turkey, soil quality of organically managed vineyards improved after 2 years of the transition period prescribed for organic certification. However, soil quality improvements in organic plots did not result in higher yield.

-Eruca sativa L. (rocket or arugula) and Diplotaxis tenuifolia L. (perennial wall-rocket)

The leafy vegetables contain high amount of mineral elements and health promoting compound. To solve nutritional problems in diet and reduced malnutrition among human population selection of specific cultivar among species would be help increasing elemental delivery in the human diet. While rocket plant observes several nutritional compounds no significant efforts have been made for genetic diversity for mineral composition of rocket plant accessions using multivariate analyses technique. The objective of this work was to evaluate variability for mineral accumulation of rocket accessions revealed by multivariate analysis to use further breeding program for achieve improving cultivar in targeting high nutrient concentration. A total twelve mineral element and twenty-three E. sativa accessions were investigated and considerable variation were observed in the most of concentration the principal component analysis explained that 77.67% of total variation accounted for four PC axis. Rocket accessions were classifies into three groups and present outcomes of experiments revealed that the first three principal components were highly valid to classify the examined accessions and separating mineral accumulations. Significant differences exhibited in mineral concentration among examined rocket accessions and the result could allow selecting those genotypes with higher elements