This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.Plant Breed. Biotech. 2015 (September) ABSTRACT A total of six markers RM3586 and RM160 on chromosome 3 and RM3735, RM3471, RM3687 and RM3536 on chromosome 4 were used to select promising lines in backcrossing populations for heat tolerance at flowering stage in rice. Fifty lines selected in BC3F2, BC4F1, and BC4F2 and parents were planted in 2013, and 2014 dry seasons at the CLRRI field under natural heat stress and greenhouse to evaluate heat tolerance at the reproductive period. Heat tolerance scoring under field condition was based on percentage of unfilled grains. All selected lines exhibited their homozygous alleles with two heat tolerance germplasm N22 or Dular in QTL loci. Twelve lines harboring homozygous alleles to QTL loci RM3586 on chromosome 3 and RM3735 on chromosome 4, respectively were selected and evaluated to agronomic traits and yield potential. Four lines BC4-1-10-1 from OM5930/N22//4 *OM5930, BC4-5-8 from OM5930/Dular//4*OM5930, BC4-5-9-4 from AS996/N22//4*AS996, and BC4-6-3 from AS996/Dular//4 *AS996, respectively were finally selected to would be for regional adaptable test in Central Coast of Vietnam under heat stress condition to release to rice farmers.
Soybean production in Vietnam has recently been threatened by the widespread dissemination of soybean rust (SBR) caused by the fungus Phakopsora pachyrhizi. Application of molecular breeding is considered as a feasible method to improve soybean rust resistance and minimize the adverse effects from overuse fungicides in this country. In this study, we have successfully applied molecular markers in a backcross breeding program to introgress the Rpp5 gene of SBR resistance into HL203, an elite Vietnamese soybean variety, from two donor lines of DT2000 and Stuart 99084B-28. The plants in BC4F1 generation had maximum contribution from the recurrent parents and retained SBR resistance gene.
Oryza rufipogon
Griff. is a perennial species of wild rice widely distributed along the channels and rivers of the Mekong Delta, Vietnam. This study attempted to find centers of diversity among wild rice populations in this area and their inter‐relationships. The highest genetic diversity was found in the Dong Thap population and the lowest in the Can Tho population. Maternal diversity evaluated using chloroplast INDELs detected ten plastid types, five of which were novel relative to other Asian countries. The mitochondrial genome suggested two unique deletions. One 699‐bp deletion via short tandem repeats was accompanied by another deletion including
orf153
. All accessions carrying the mitochondrial type were found in a particular plastid type. This unique maternal lineage was confined to specific channels where it showed vigorous vegetative growth in comparison to upstream areas where various maternal lineages and maximum genetic diversity occurred. This area along the Mekong Delta is a center of not only nuclear but also maternal diversity.
To increase productivity and improve quality, new rice varieties need to be salinity tolerance and resistant to many kinds of insects, diseases, and high levels of nutrients, resistant to disadvantageous conditions, and promises to reduce using pesticides, chemical fertilizers. Great efforts have been focused on germplasm research to discover genes resistant to disease and insect, efficient in using salinity level with good grain quality and productivity. With the development of climate-resilient varieties, scaling up or dissemination of seeds is done by province, following the locality's is Winter-Spring (DongXuan Season) and Summer -( Wet season ). For instance, in the case of Winter-Spring crop, the plan of Mekong delta is to expand some lines such as HATRI 190, HATRI 192, HATRI 170were developed that can yield4-5 tons ha -1 under salt stress of 10.0 to 12.0 dS m -1 , and are being out-scaled. Normally, this expansion initiative requires inclusion of key rice varieties, that is, local or extra varieties and promising varieties, all of which will be included in the plan for developing the rice sector for each province.
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