Applying Molecular Breeding to Improve Soybean Rust Resistance in Vietnamese Elite Soybean

Abstract

<span><span style="color:black;">Soybean production in </span></span></a><span><span><span style="color:black;">Vietnam</span></span></span><span><span><span style="color:black;"> has recently been threatened by the widespread </span></span></span><span style="color:black;">dissemination of soybean rust (SBR) caused by the fungus <i>Phakopsora pachyrhizi</i>. Application of molecular breeding is considered as a feasible method to improve soybean rust resistance and minimize the adverse effects from overuse fungicides in this country. In this study, we have successfully applied molecular markers in a backcross breeding program to introgress the <i>Rpp</i>5 gene of SBR resistance into HL203, an elite Vietnamese soybean variety, from two donor lines of DT2000 and Stuart 99084B-28. The plants in BC₄F₁ generation had maximum contribution from the recurrent parents and retained SBR resistance gene.</span><span style="color:black;"></span

Breeding cowpea for resistance to Striga gesnerioides in the Nigerian dry savannas using marker‐assisted selection

“…This result corroborates the work of Khanh et al. () who applied molecular markers to improve soybean for rust resistance with high precision of selection. The only discrepancy observed was that the progeny earlier phenotypically classified as resistant were found to be susceptible to Striga parasitism on the field.…”

“…A number of crop varieties or breeding lines such as soybean (Khanh et al. ), sorghum (Gamar and Mohammend ) and cowpea (Ouédraogo et al. ) have been developed using the MAS approach which shortened the breeding cycle considerably.…”

“…Therefore, application of MAS for efficient selection systems would fast-track the breeding efforts to introgress Striga-resistant gene(s) into locally adapted cowpea cultivars targeted for Striga prone areas. A number of crop varieties or breeding lines such as soybean (Khanh et al 2013), sorghum (Gamar and Mohammend 2013) and cowpea (Ou edraogo et al 2002) have been developed using the MAS approach which shortened the breeding cycle considerably. The objective of this study was to improve cowpea productivity in major cowpea growing areas in northern Nigeria that are highly infested with S. gesnerioides.…”

Translational Genomics in Agriculture: Some Examples in Grain Legumes

“…By using 2-3 rounds of backcrossing and selfing, BC 2 F 3 and BC 3 F 2 homozygous lines have been developed at ICRISAT. (Varshney et al, 2013c) Aschochyta blight resistance Back cross progenies from (C 214 × ILC 3279) (Varshney et al, 2013c) Drought tolerance Back cross progenies from (JG 11 × ICC 4958) (Varshney et al, 2013b) Back (Smith et al, 2010) Rust resistance Back cross lines (Khanh et al, 2013) Two major MABC projects are underway in chickpea at ICRISAT and its partner organizations. Under the Tropical Legume-I initiative of CGIAR Generation Challenge Programme in collaboration with Bill & Melinda Gates Foundation, significant efforts have been made to develop drought tolerant progenies (BC 3 F 3:4 ) in the genetic background of JG11, a leading variety in India, by transferring a genomic region, "QTLhotspot," that contains several QTLs for drought tolerance traits (Varshney et al, 2013b).…”

Effect of Different Growth Regulators on Soybean (Glycine max L.) Regeneration

“…Drought stress affects all stages of plant growth and consequently reduces the crop yield (Manavalan et al, 2009). An effort has been made using classical breeding approaches to develop the disease (Khanh et al, 2013) and pest resistant varieties (Dar et al, 2006) but due lack of resources, none of the drought resistant variety has been developed yet in India. As the transformation techniques are well In present study efforts were made to improve the regeneration protocol in the popular local soybean cultivars MAUS-71 and MAUS-162.…”