Few studies have evaluated fecal indices for monitoring diet quality and intake of North American deer. We conducted 11 digestion trials with black-tailed (Odocoileus hemionus columbianus Richardson) and mule deer (0. h. hemionus Rafmesque) to examine relationships between several chemical constituents of deer feces (i.e., fecal nitrogen, fecal 2,6diaminopimelic acid (DAPA), fecal neutral detergent fiber (NDF), fecal acid detergent fiber, and fecal acid detergent lignin) and dry matter intake, digestible energy, digestible energy intake, diet crude protein content, crude protein digestibility, and digestible crude protein intake. We developed regression equations to predict diet quality and intake and also evaluated 2 alternative methods (organic matter basis and neutral detergent fiber (ndt) basis) for quantifying fecal indices. Concentrations of DAPA, fecal NDF, and fecal N were the most precise for estimating diet quality and intake. Extracts from 5 of 11 diets precipitated only small amounts of protein and influence of tannins on protein digestion probably was slight. Quantifying fecal indices per unit organic matter and neutral detergent fiber in the feces was comparable to the standard dry matter basis and under some field conditions should improve their predictive ability. We believe our best equations are suitable for management purposes where diets are similar and intake and quality are believed to be within the ranges we documented.
Laboratory scale preparation of bovine serum albumin (BSA) stained with Coomassie brilliant blue (CBB) at alkaline pH is first described. Physical-chemical analyses of CBB-BSA showed that the unprotonated (anion) CBB dye binds tightly to BSA in buffered media of pH 8.2. Characteristic differences in spectra lambda(max) and molar absorptivities were found for the free anion CBB dye versus the CBB-BSA complex. Binding studies with low versus high dye/protein concentration ratios at alkaline pH gave values for n, binding site numbers, and K, intrinsic binding coefficient, consistent with those reported in analytical studies under acidic pH, but higher than values for neutral pH. Comparative analyses of Beer's law plots for the alkaline CBB-BSA complex under different experimental conditions showed its high stability toward various interferences, such as pH, strong detergents, temperature, light, prolonged storage, as well as high affinity for tannins. The hydrophobic nature of the CBB-BSA association at alkaline pH was tested.
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