The potential use of stable-isotope analyses (6I3C and 6I5N) to estimate bear diets was assessed in 40-day feeding trials using American black bears (Ursus americanus). Bear plasma and red blood cells have half-lives of -4 days and -28 days, respectively. The isotopic signature of bear plasma is linearly related to that of the diet, and with the exception of adipose tissue, there is no isotopic fractionation across bear tissues. Isotopic analyses were used to estimate the diets of three bear populations: Pleistocene cave bears (U. speleaus) in Europe, grizzly bears (Ursus arctos horribilis) inhabiting the Columbia River drainage prior to 1931, and brown bears (U. arctos) of Chichagof and Admiralty islands, Alaska. Cave bears were omnivores with terrestrially produced meat contributing from 4.1 to 78% (58 f 14%) of their metabolized carbon and nitrogen. Salmon contributed from 33 to 90% (58 f 23%) of the metabolized carbon and nitrogen in grizzly bears from the Columbia River drainage. Finally, most brown bears on Chichagof and Admiralty islands feed upon salmon during the late summer and fall; however, a subpopulation of bears exists that does not utilize salmon.
We tested the hypothesis that tannins defend plants against large herbivores by decreasing protein availability. Digestion trials were conducted with mule deer (Odocoileus hemionus) and results from previous trials with white—tailed deer (O. virginianus), moose (Alces alces), caribou/reindeer (Rangifer tarandus), and elk (Cervus elaphus) were summarized to evaluate dietary factors affecting protein availability. The digestibility of plant protein in feeds with minimal tannins, such as grasses and agriculturally produced legumes and grains, was a highly predictable function of the total protein content and the amount of nondigestible, fiber—bound protein. Digestible protein in plants containing significant tannins was lower than predicted from regressions for low—tannin feeds. The reduction in digestible protein was proportional to the protein—precipitating capacity of the plant tannins. Deciduous browse stems collected in winter had very low levels of protein—precipitating tannins and only a slightly lower protein availability than predicted. Tannins are not important in the defense of most deciduous tree shrub stems consumed by these herbivores. Tannins in flowers and forb, tree, and shrub leaves markedly reduced protein availability. Tannins must be considered in understanding the defensive strategies of leaves and flowers. Voluntary intake of the high—phenolic forages was significantly reduced below ingestion rates for grasses, legumes, and pelleted diets. It is hypothesized that soluble phenolics that do not inhibit digestion but are absorbed and reduced intake through their toxicity are more important in defending some plant parts against ruminants than are digestion—reducing tannins.
We tested the competing hypotheses that (1) nitrogen discrimination in mammals and birds increases with dietary nitrogen concentration or decreasing C:N ratios and, therefore, discrimination will increase with trophic level as carnivores ingest more protein than herbivores and omnivores or (2) nitrogen discrimination increases as dietary protein quality decreases and, therefore, discrimination will decrease with trophic level as carnivores ingest higher quality protein than do herbivores. Discrimination factors were summarized for five major diet groupings and 21 different species of birds and mammals. Discrimination did not differ between mammals and birds and decreased as protein quality (expressed as biological value) increased with trophic level (i.e., herbivores to carnivores). Relationships between discrimination factors and dietary nitrogen concentration or C:N ratios were either the opposite of what was hypothesized or non-significant. Dietary protein quality accounted for 72% of the variation in discrimination factors across diet groupings. We concluded that protein quality established the baseline for discrimination between dietary groupings, while other variables, such as dietary protein intake relative to animal requirements, created within-group variation. We caution about the care needed in developing studies to understand variation in discrimination and subsequently applying those discrimination factors to estimate assimilated diets of wild animals.
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