Complex mixtures of unknown contaminants present a challenge to identify toxicological risks without using large numbers of animals and labour-intensive screens of all organs. This study examined soil extracts from a legacy contaminated pesticide packaging and blending site. HepG2 cytotoxicity was used as an initial screen of 18 soil samples to identify the most toxic extracts for testing in animals. Then, target organ toxicities were identi ed following biweekly oral gavage for one month of three soil extracts (0.1% in polyethylene glycol or PEG) compared to vehicle control in male Sprague Dawley rats (n = 9-10/group). Exposure to extract A signi cantly increased neutrophils and lymphocytes compared to control. In contrast, all extracts increased plasma α-2 macroglobulin and caused mild to moderate lymphocytic proliferation within the spleen white pulp, all indicative of in ammation. Rats exposed to all soil extracts exhibited acute tubular necrosis. Cholinesterase activity was signi cantly reduced in plasma, but not brain, after exposure to extract A compared to control. Increased hepatic ethoxyresoru n-odeethylase activity compared to control was observed following exposure to extracts A and B. Exposure to soil extract C in rats showed a prolonged QTc interval in electrocardiography as well as increased brain lipid peroxidation. Candidate contaminants are organochlorine, organophosphate/carbamate pesticides or metabolites. Overall, HepG2 cytotoxicity did not successfully predict the neurotoxicity and cardiotoxicity observed with extract C, but was more successful with suspected hydrocarbon toxicities in extracts A and B. Caution should be taken when extrapolating the observation of no effects from in vitro cell culture to in vivo toxicity and better cell culture lines or assays should be explored.

Complex mixtures of unknown contaminants present a challenge to identify toxicological risks without using large numbers of animals and labour-intensive screens of all organs. This study examined soil extracts from a legacy contaminated pesticide packaging and blending site. HepG2 cytotoxicity was used as an initial screen of 18 soil samples to identify the most toxic extracts for testing in animals. Then, target organ toxicities were identified following biweekly oral gavage for one month of three soil extracts (0.1% in polyethylene glycol or PEG) compared to vehicle control in male Sprague Dawley rats (n = 9–10/group). Exposure to extract A significantly increased neutrophils and lymphocytes compared to control. In contrast, all extracts increased plasma α-2 macroglobulin and caused mild to moderate lymphocytic proliferation within the spleen white pulp, all indicative of inflammation. Rats exposed to all soil extracts exhibited acute tubular necrosis. Cholinesterase activity was significantly reduced in plasma, but not brain, after exposure to extract A compared to control. Increased hepatic ethoxyresorufin-o-deethylase activity compared to control was observed following exposure to extracts A and B. Exposure to soil extract C in rats showed a prolonged QTc interval in electrocardiography as well as increased brain lipid peroxidation. Candidate contaminants are organochlorine, organophosphate/carbamate pesticides or metabolites. Overall, HepG2 cytotoxicity did not successfully predict the neurotoxicity and cardiotoxicity observed with extract C, but was more successful with suspected hydrocarbon toxicities in extracts A and B. Caution should be taken when extrapolating the observation of no effects from in vitro cell culture to in vivo toxicity and better cell culture lines or assays should be explored.

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