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The monitoring and control of bioprocesses is of the utmost importance in order to provide a consistent, safe, and high-quality product for consumers. Current monitoring and control schemes rely on infrequent and time consuming offline sampling methods, which inherently leads to some variability in the process which may impact the product quality profile. As part of Lonza's dedication to process analytical technology (PAT) initiatives this study evaluated the ability to generate generic calibration models, which are independent of the cell line, using Raman probes to monitor changes in glucose, lactate, glutamate, ammonium, viable cell concentration (VCC), total cell concentration (TCC) and product concentration. Calibration models were developed from cell culture using two different CHOK1SV GS-KO cell lines producing different monoclonal antibodies (mAbs). Developed predictive models, measured changes in glucose, lactate, ammonium, VCC, and TCC with average prediction errors of 0.44, 0.23, 0.03 g L , 1.90 × 10 cells mL , and 1.85 × 10 cells mL , respectively over the course of cell culture with minimal cell line dependence. The development of these generic models allows the application of spectroscopic PAT techniques in clinical and commercial manufacturing environments, where processes are typically run once or twice in GMP manufacturing based on a common platform process. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:730-737, 2018.

It is one thing to have a discussion or write about a one-or two-foot rise in the ocean surface and potential impacts to a local community; it is another to show someone a map highlighting the areas that would potentially be impacted. The ability to visualize the potential depth and inland extent of water gives us a better understanding of the corresponding impacts and consequences. Mapping sea level changes in a geographic information system (GIS) gives the user the ability to overlay the potentially impacted areas with other data such as critical infrastructure, roads, ecologically sensitive areas, demographics, and economics. Providing maps on the Web via Internet mapping technologies enables the user to have an interactive experience that truly brings out the "visual" part of the map definition. Over the past several years, the lessons learned from investigating pilot sea level change mapping applications have led to the development of a next-generation sea level rise and coastal flooding viewer. In addition, new mapping techniques have been developed to use high-resolution data sources to show flooding impacts on local public infrastructure, mapping confidence, flooding frequency, marsh impacts, and social and economic impacts from potential inundation. This paper will provide a brief history of previous sea level change visualization pilot projects, detailed discussion of new methods, current status of new tool development and outputs, and future plans for expanding to the rest of the U.S.

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