Adipose tissue has been shown to contain adult mesenchymal stem cells that have therapeutic applications in regenerative medicine. There is evidence that the ability of adipose precursor cells to grow and differentiate varies among fat depots and changes with age. Defining these variations in cell function and molecular mechanisms of adipogenesis will facilitate the development of cellbased therapies. We compared cells harvested from 5 different subcutaneous (SC) adipose depots in 12 female patients classified into 3 age ranges (25-30, 40-45, and 55-60 years old). Capacity for differentiation of isolated adipose-derived stem cells (ASCs) with and without ciglitazone, a strong peroxisome proliferatoractivated receptors (PPAR)-γ agonist, was assessed in vitro. ASCs were also characterized by lipolytic function, proliferation, and sensitivity to apoptosis. Additionally, PPAR-γ-2 protein expression was determined. We observed a difference in the apoptotic susceptibility of ASCs from various SC depots, with the superficial abdominal depot (above Scarpas layer) significantly more resistant to apoptosis when compared with the 4 other depots. We have also demonstrated that a PPAR-γ agonist aids in the induction of differentiation in cells from all depots and ages. Although sensitivity to apoptosis was linked to anatomic depot, differences in cell proliferation were related primarily to age. Stimulated free glycerol release has been shown to be highest in the arm depot. The arm depot has also consistently shown expression of PPAR-γ-2 with and without a PPAR-γ agonist. Younger patients have increased PPAR-γ-2 expression in all depots, whereas the older patients have consistent elevated expression only in the arm and thigh depots. We have shown there is variability in function of ASCs that have been harvested from different SC depots. Additionally, we have shown age-related changes in function. These data will help select patients and cell harvest sites most suitable for tissue engineering therapies.

Small intestinal submucosa particles are a favorable scaffold for preadipocytes, allowing ex vivo proliferation on particles small enough to be injected. Delivery of FGF-2 from poly(lactic-co-glycolic acid) microspheres resulted in cell survival and enhanced vascularization.

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Introduction The COVID-19 pandemic has compelled a majority of hospital systems to reduce surgical and procedural volumes in an attempt to preserve resources. Elective surgery and procedures (ESP) resumption has proven to be a calculated risk between COVID-19 exposure and resource depletion and patient morbidity and mortality from surgical deferral. Methods Within a few days of halting ESP, our 7-hospital (2427 in-patient beds, 26,647 inpatient surgeries) healthcare system developed a multidisciplinary Pivot Plan with the primary outcome of a phased resumption of ESP. The plan entailed the integration of our electronic medical record, order entry automatization, perioperative staff utilization, partnering with primary care providers, and a step-wise COVID-19 testing algorithm based on a pre-determined hierarchy of case acuity and timeliness of patient care. Results The Pivot Plan was instituted on May 10, 2020. Since then, 22,624 patients have been tested for COVID-19 in anticipation of an ESP; 140 (0.62%) tested positive for COVID-19 and had their procedure deferred. As our testing capability has increased, we have been able to increase our added ESP capacity from 13 cases per day to 531 cases per day. In turn, we have seen the case volume increase by 52%. Conclusion Our academic healthcare system located in one of the initial COVID − 19 hotspots in the United States has successfully resumed ESP in part due to a receptive and supportive culture, based upon nimbleness, agility, and rapid integration of multiple resources from a cohort of diverse disciplines applied to the perioperative services workflow.

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not available P5. MECHANISMS OTHER THAN ACUTE HYPERGLYCEMIA MEDIATE ENDOTHELIAL CELL ACTIVATION IN SYS-TEMIC INFLAMMATORY RESPONSES.Abstract not available P6. SHEAR FORCE UP-REGULATES ENDOTHELIAL CELL MARKERS IN HUMAN ADIPOSE-DERIVED STEM CELLS.Abstract not available P7.Abstract not available P8. ENVIRONMENTAL FACTORS DETERMINE IN VIVO MYOGENIC PROGENITOR CELL FUNCTION.Abstract not available P9.Abstract not available P10. MITOCHONDRIAL DEFECTS AND OXIDATIVE DAMAGE IN PATIENTS WITH PERIPHERAL ARTERIAL DISEASE. Iraklis I.Abstract not available P11. IDENTIFICATION OF MESSENGER RNA SPECIES WITH INTERNAL RIBOSOME ENTRY SITES IN VIRALLY INFECTED HUMAN ENDOTHELIAL CELLS.Abstract not available P12. OMEGA-3 FATTY ACID SUPPLEMENTATION MODU-LATES THE INFLAMMATORY RESPONSE IN PATIENTS WITH TRUAMATIC SHOCK. Abstract not availableAbstract not available P14.Abstract not available P15. AORTIC CALCIFICATION IN C3H MICE IS PRE-VENTED BY ELASTIN STABILIZATION. Raul J. Guzman, Paul M. Schumacher, Chance Irwin; Vanderbilt University Medical Center, Nashville, TN Abstract not available P16. SURFACTANT DYSFUNCTION IN LUNG CONTUSION CORRELATES WITH SEVERITY OF LUNG INJURY.Abstract not available P17. ADMINISTRATION OF CLODRONATE CONFERS ADDED PROTECTION AGAINST HALR.Abstract not available P18. ELDERLY LUNG CANCER PATIENTS HAVE THE SAME SURVIVAL WHETHER TREATED WITH LOBECTOMY OR WEDGE RESECTION.Abstract not available 447 ASSOCIATION FOR ACADEMIC SURGERY AND SOCIETY OF UNIVERSITY SURGEONS-ABSTRACTS