In the search for improved yields, seed treatment by microbiolization has been used as an alternative to chemical treatment. The objective was to verify the physiological and sanitary quality of creole bean seeds, var. Chumbinho, after microbiolization with doses of a commercial product (c.p.) with Trichoderma harzianum (strain ESALQ-1306). The treatments were: T1) 100 mL c.p./100 kg seeds; T2) 150 mL c.p.; T3) 200 mL c.p.; T4) 200 mL of chemical treatment (c.p., 250 g L-1 fipronil + 25 g L-1 pyraclostrobin + 225 g L-1 thiophanate-methyl); and T5) control (without coating of seeds). The tests were: sanitary test (blotter test); germination and first count; accelerated aging, cold germination without soil, speed of germination rate (SGR), seedling shoot and root lengths, and emergence of seedlings in a greenhouse. T. harzianum controlled Aspergillus spp., Penicillium spp. and Fusarium oxysporum. With 100 mL c.p. of T. harzianum dose had better results for the germination and vigor, and this dose it is an alternative to chemical treatment in creole bean seeds.

Considering the importance of bean cultivation, the objective was to prove the effectiveness of the seed microbiolization method in the control of Sclerotinia sclerotiorum (Ss) on black bean seeds (cv. IPR Tuiuiú), inoculated by the water restriction method. The treatments were fungicide methyl thiophanate + fluazinam (350.0 g L-1 + 52.5 g L-1); Trichoderma asperellum BV-10 (1.0 x 1010 viable conidia mL-1); T. harzianum strain CCT 7589 (1 x 109 CFUs L-1); Bacillus subtilis BV-02 (minimum 3.0 x 109 CFU mL-1); B. amyloliquefaciens isolated BV03 (minimum 3.0 x 109 CFU L-1); Positive control (seeds exposed to Ss); Negative control (NC) + fungicide; NC + T. asperellum; NC + T. harzianum; NC + B. subtilis; NC + B. subtilis; and NC (PDA medium plus restricting). To verify the effects on germination, vigor, and the health of bean seeds, and seedling emergence in the field. Microbiolization with T. asperellum and T. harzianum, provides improvement in germination, vigor, and health, but is not superior to fungicide treatment. Bacillus subtilis and B. amyloliquefaciens, in seeds not inoculated with the pathogen, produce the best results for green and dry mass (g). These may be used in the integrated management of white bean mold.

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Seed priming is a treatment that can contribute to improve seed physiological potential and increase its tolerance to abiotic stresses. Thus, this work evaluated the effect of kale seed priming with red seaweed biostimulant on physiological seed potential, seed health and tolerance to high temperature at germination. The experimental design was completely randomised, with a 2´x4 factorial scheme. Treatments consisted of doses of 0, 0.25, 0.50 and 1.0 mL L–1 of red algae Solieria sp. biostimulant, and temperatures of 20 and 30°C. The biostimulant used was subjected to chromatographic analysis to detect bioactive compounds. Seed imbibition curves were used to determine priming duration procedure. Treatments effects were evaluated by seed health, germination, root and shoot length, and dry mass, under ideal (20°C) and stress (30°C) temperatures. The results were submitted to analysis of variance, Tukey’s test (temperatures) and regression (doses). The 22-h imbibition period is adequate for kale seed priming with Solieria sp. biostimulant. Kale seed priming with Solieria sp. does not interfere with seed health. The temperature of 30°C reduces kale seed germination index, as seedling root growth. The use of Solieria sp. biostimulant does not promote kale seed physiological potential.

A temperatura é um fator que interfere diretamente na capacidade germinativa de sementes. Com as elevações de temperaturas ocasionadas pelo aquecimento global, assim como em situações de cultivo protegido de plantas em ambientes tropicais e subtropicais, é necessário estudar procedimentos que possam contribuir para aumento da tolerância a temperaturas que causam estresse e alteração em processos fisiológicos. Desta forma, o objetivo desse trabalho foi avaliar o efeito do condicionamento fisiológico de sementes de couve com diferentes doses de extrato da alga marrom Ascophyllum nodosum na germinação e crescimento de plantas em diferentes temperaturas. Realizou-se o experimento em delineamento experimental inteiramente casualizado, com esquema fatorial 2 x 4 (temperaturas x doses), com cinco repetições. Os tratamentos consistiram das doses de 0; 0,25; 0,50; 1,0 ml. L-1 de extrato da alga marrom Ascophyllum nodosum. O efeito dos tratamentos foi avaliado por meio de: porcentagem e velocidade de germinação, comprimento e massa seca da parte aérea e de raízes de plântulas, em condições de temperatura ideal (20°C) e de estresse (30°C). O condicionamento de sementes de couve com extrato da alga marrom Ascophyllum nodosum não interfere na germinação de sementes, tanto em condição ideal quanto em temperatura elevada (30°C); o condicionamento de sementes de couve com as doses de 0,67 e 0,25 mL.L-1, promove maior crescimento de raízes de plântulas, nas temperaturas de germinação de 20 e 30°C, respectivamente, contudo, não promove incrementos no crescimento de parte aérea e no acúmulo de massa seca total de plântulas.