The aim of the present study was to investigate the effect of resveratrol on apoptosis in SGC-7901 gastric cancer cells and its molecular mechanisms of action. Following resveratrol treatment, the inhibition rate of SGC-7901 cells was determined using an MTT assay. The morphological changes in apoptosis were observed by fluorescence microscopy based on acridine orange/ethidium bromide double staining. Furthermore, cell cycle and apoptosis were detected using flow cytometry, and the expression levels of nuclear factor κB (NF-κB) as well as apoptosis-associated proteins [B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X protein (Bax), cleaved caspase-3 and cleaved caspase-8] were analyzed by western blotting. The results of the present study indicated that resveratrol was able to significantly inhibit the viability of SGC-7901 cells in a dose- and time-dependent manner. When treated with 200 µM resveratrol, the inhibition rate of SGC-7901 cells reached ~50%. In the presence of resveratrol, the proportion of apoptotic cells was also increased in a dose-dependent manner. Flow cytometry revealed that resveratrol induced S-phase arrest of SGC-7901 cells. When treated with 50, 200 and 400 µM resveratrol, the proportions of SGC-7901 cells in the S-phase were respectively increased to 33.8±2.42, 60.01±2.43 and 56.05±2.67%, compared with 25.62±3.29% for the control group cells in S-phase. Additionally, the levels of the pro-apoptotic proteins Bax, cleaved caspase-3 and cleaved caspase-8 were upregulated in a dose-dependent manner, whereas the level of the anti-apoptotic protein Bcl-2 was downregulated dose-dependently. Importantly, the activation of NF-κB (p65) was evidently decreased following treatment with resveratrol compared with in the control group. In conclusion, the results of the present study revealed that resveratrol was able to inhibit viability and induce apoptosis in SGC-7901 cells by suppressing NF-κB activation. Therefore, resveratrol may be considered as a potential drug candidate for the treatment of gastric cancer.
The
novel brominated flame retardant decabromodiphenyl ethane (DBDPE)
has become a widespread environmental pollutant. However, the target
tissue and toxicity of DBDPE are still not clear. In the current study,
female zebrafish were exposed to 1 and 100 nM DBDPE for 28 days. Chemical
analysis revealed that DBDPE tended to accumulate in the brain other
than the liver and gonad. Subsequently, tandem mass tag-based quantitative
proteomics and parallel reaction monitoring verification were performed
to screen the differentially expressed proteins in the brain. Bioinformatics
analysis revealed that DBDPE mainly affected the biological process
related to muscle contraction and estrogenic response. Therefore,
the neurotoxicity and reproductive disruptions were validated via
multilevel toxicological endpoints. Specifically, locomotor behavioral
changes proved the potency of neurotoxicity, which may be caused by
disturbance of muscular proteins and calcium homeostasis; decreases
of sex hormone levels and transcriptional changes of genes related
to the hypothalamic-pituitary-gonad-liver axis confirmed reproductive
disruptions upon DBDPE exposure. In summary, our results suggested
that DBDPE primarily accumulated in the brain and evoked neurotoxicity
and reproductive disruptions in female zebrafish. These findings can
provide important clues for a further mechanism study and risk assessment
of DBDPE.
A novel brominated flame retardant decabromodiphenyl ethane (DBDPE) has become a ubiquitous emerging pollutant; hence, the knowledge of its long-term toxic effects and underlying mechanism would be critical for further health risk assessment. In the present study, the multi-and transgenerational toxicity of DBDPE was investigated in zebrafish upon a life cycle exposure at environmentally relevant concentrations. The significantly increased malformation rate and declined survival rate specifically occurred in unexposed F2 larvae suggested transgenerational development toxicity by DBDPE. The changing profiles revealed by transcriptome and DNA methylome confirmed an increased susceptibility in F2 larvae and figured out potential disruptions of glycolipid metabolism, mitochondrial energy metabolism, and neurodevelopment. The changes of biochemical indicators such as ATP production confirmed a disturbance in the energy metabolism, whereas the alterations of neurotransmitter contents and light−dark stimulated behavior provided further evidence for multi-and transgenerational neurotoxicity in zebrafish. Our findings also highlighted the necessity for considering the long-term impacts when evaluating the health of wild animals as well as human beings by emerging pollutants.
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