Unraveling
the changing rule of endoplasmic reticulum (ER) polarity
is of significance for liver injury. However, the rule of the ER polarity
changes during the occurrence and progression of liver injury remains
a mystery. Toward that, a unique fluorescent probe, ERNT, capable of imaging ER polarity in multiple liver injury models
with high accuracy and fidelity was designed herein. In light of its
excellent solvatochromism, the ER polarity was determined to be higher
in the case of endoplasmic reticulum stress (ERS) induced by tunicamycin
and dithiothreitol than that of the normal state at the cell level.
Importantly, with the assistance of the PerkinElmer IVIS Spectrum
imaging system and the powerful tool of ERNT, our work
first revealed that the ER polarity increases with the evolution of
liver injuries. Moreover, as a demonstration, ERNT achieved
evaluating hepatoprotective drug efficacy by detecting ER polarity,
confirming its high clinical application prospect. Thus, our work
not only first unravels the rule of ER polarity in dynamic liver injury
progression but may also inspire more diagnostic and therapeutic programs
for liver diseases shortly.
Exploring the changes in the polarity of intracellular lipid droplets (LDs) during the evolution of cancer is of great significance for cancer detection and treatment. For this reason, we designed...
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