Lipid droplet polarity decreases during the pathology of muscle injury as revealed by a polarity sensitive sensor

Lipid Droplet-Specific Dual-Response Fluorescent Probe for the Detection of Polarity and H2O2 and Its Application in Living Cells

“…48 As for polarity detection, probes are usually constructed by D−π−A type organic fluorescent dyes with the ICT effect, 49−51 which display low fluorescence intensity in strong polar medium, but weakly polar regions within cells can be imaged (membranes and lipid droplets), so compound 1 is chosen to image polarity to avoid spectral overlap with naphthalimide. Therefore, NATPA is designed and synthesized by the route shown in Scheme S1, and its structure is characterized by 1 H NMR, 13 C NMR, and HRMS (ESI). The fluorescence spectrum was studied to further evaluate the ability of NATPA to recognize H 2 O 2 (Figure 1).…”

“…Hydrogen peroxide (H 2 O 2 ), the most stable endogenous reactive oxygen species, is mainly generated in mitochondria and serves as an important messenger molecule in the cell cycle. − The overexpression of intracellular H 2 O 2 is associated with diverse diseases. − Polarity, a vital microenvironmental parameter, plays a key role in regulating biochemical reactions in cells. , It also markedly interferes with the interaction of proteins and enzymes as well as the permeability of cell membranes, so abnormal cell polarity also induces various diseases. − The polarity of lipid droplets (LDs) reflects its features and function and also presents a considerable effect on adjusting biological processes in cells, such as lipid metabolism, membrane transport, and protein–protein interactions. − So far, there have been some probes reported for imaging LD polarity. − Therefore, it is of great significance to monitor intracellular H 2 O 2 and polarity fluctuations. As is well known, there is a close connection between H 2 O 2 and polarity.…”

A Solvatochromic Fluorescent Probe for Solvent Polarity Detection Using a Smartphone

“…[23][24][25][26] Several solvent chromotropic fluorescent probes have been developed to explore the solvent polarity, most of which display obvious diversity in spectral intensity and wavelength measurement. [27][28][29][30][31][32][33][34][35][36][37][38][39] The polarities of pure solvent can be determined by comparing the linear relationship between fluorescence emission wavelength or Stokes shift with polarity parameters. [40,41] The RGB (Red, Green, Blue) is an additive color model in which red, green, and blue primary colors of light are added in various ways to produce a specific color.…”

“…Out of the solvent polarity parameter detection methods, fluorescent probe detection has become a promising method because of its ease of operation, high sensitivity, and selectivity [23–26] . Several solvent chromotropic fluorescent probes have been developed to explore the solvent polarity, most of which display obvious diversity in spectral intensity and wavelength measurement [27–39] . The polarities of pure solvent can be determined by comparing the linear relationship between fluorescence emission wavelength or Stokes shift with polarity parameters [40,41] .…”

Dynamic Excimer (DYNEX) Imaging of Lipid Droplets

“…Additionally, microenvironment polarity is an intrinsically complex parameter involving the medium acting as a continuous phase and specific molecular interactions of dispersion forces, dipole-dipole interactions, and hydrogen bonds, affecting enzymatic activity, membrane transport, and cellular proliferation . Thus, microenvironment polarity inside lipid droplets reveals different cellular metabolic states, either physiological or pathological. − …”