Purpose -The purpose of this paper is to report a study about the rapid prototyping method of dental glass-ceramic restoration. Design/methodology/approach -Dental glass-ceramic restoration materials have excellent physical and chemical, mechanical, aesthetic and biocompatibility characteristics. However, casting methods adopted at present have complicated procedures and high costs; the forming qualities are especially difficult to control. These problems greatly restrict their clinical application and promotion. Therefore, a new forming process based on selective laser sintering (SLS) technology is proposed. First, dental glass-ceramic is processed into fine powder through a special heat treatment process. Then, the dental restoration parts are manufactured using SLS without any moulds. In this paper, the effects of processing parameters including laser power, scan speed, scan spacing and preheating temperature on the relative density and mechanical properties of the sintered parts are studied. Findings -The experimental results have shown that for the composite powder of epoxy resin binder E-12 and K 2 O-Al 2 O 3 -SiO 2 series of dental glassceramics, when preheating temperature, layer thickness, laser power, scan speed and scan spacing are, respectively, 30 , 358C, 0.08 mm, 21 W, 1,800 mm/s and 0.10 mm/s, the relative densities of dental glass-ceramic parts are relatively high; the mechanical properties and forming effect are excellent. The relative density and bending strength of SLS parts under the optimized processing parameters are 37.40 per cent and 2.08 MPa, respectively.Research limitations/implications -This study only concerns the preparation and SLS of the dental glass-ceramic powders. Further investigations are planned to be conducted on post processing, such as binder decomposition, isostatic press and high temperature sintering. Originality/value -This study will provide a theoretical and technical basis for dental glass-ceramic restorations of SLS.

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As a rich source of CD4+ T cells and macrophages, the gastrointestinal (GI) tract is a major target site for HIV infection. The interplay between GI-resident macrophages and intestinal epithelial cells (IECs) constitutes an important element of GI innate immunity against pathogens. In this study, we investigated whether human IECs have the ability to produce antiviral factors that can inhibit HIV infection of macrophages. We demonstrated that IECs possess functional toll-like receptor 3 (TLR3), the activation of which resulted in induction of key interferon (IFN) regulatory factors (IRF3 and IRF7), IFN-β, IFN-λ, and CC chemokines (MIP-1α, MIP-1β, RANTES), the ligands of HIV entry co-receptor CCR5. In addition, TLR3-activated IECs release exosomes that contained the anti-HIV factors, including IFN-stimulated genes (ISGs: ISG15, ISG56, MxB, OAS-1, GBP5, and Viperin) and HIV restriction miRNAs (miRNA-17, miRNA-20, miRNA-28, miRNA-29 family members, and miRNA-125b). Importantly, treatment of macrophages with supernatant (SN) from the activated IEC cultures inhibited HIV replication. Further studies showed that IEC SN could also induce the expression of antiviral ISGs and cellular HIV restriction factors (Tetherin and APOBEC3G/3F) in HIV-infected macrophages. These findings indicated that IECs might act as an important element in GI innate immunity against HIV infection/replication.

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