Background:
The use of herbal medicine in inflammatory bowel disease (IBD) had been increased significantly. Allopathic treatment of IBD leads too many side effects therefore use of the herbal formulation is promising. Aegle Marmelos, Bombax malabericum, and Hollarrhena antidysentrica plants have been used to treat IBD.
Objective:
To evaluate a designed polyherbal formulation in experimentally induced inflammatory bowel disease in rats and To validate mathematical model derived by Box-Behnken experimental design for optimized polyherbal formulation for the treatment of IBD in experimental rats by checkpoint analysis.
Method:
Three-level Box-Behnken design was selected to optimize the dose. Polyherbal formulation consist of plant extract of Aegle Marmelos (X1), Bombax malabericum (X2), and Hollarrhena antidysentrica (X3) in different ratios were selected as independent variable. Polynomial equations were established based on Analysis of variance (ANOVA). To validate the chosen polynomial equation checkpoint analysis were performed. The percentage of predictive error is presented.
Results:
ANOVA reveals that X2 plant does not have any significant impact on the response surface. The checkpoint batch showed the experimental value of CMDI and Disease activity index (DAI) as 1.33 and 0.66 respectively. It is worthwhile to note that the observed values were quite close to the calculated values of CMDI. A little difference in the value of DAI may be attributed to the inherent variation observed in animal studies.
Conclusion:
From this study, it was concluded that a dose of Aegle marmelos 100 mg/kg, a dose of Bombax malabericum 300 mg/kg, and a dose of Holarrhena antidysentrica 200 mg/kg will always be effective in IBD patients.
In present study, we evaluated the antiulcer activity of the herbal preparation of Caesalpinia crista in rat models. Experimental animals were divided into four groups. Rats of group I (disease control) treated with normal saline only, group II (standard group) treated with Omeprazole (2 mg/ kg; p.o.), group III and IV served as test groups and were treated with Caesalpinia crista extract (CE) in the dose of 250 mg/ kg and 500 mg/ kg orally respectively. Peptic ulcer was induced by ligating the pyloric portion of rat stomach and was done 45 min after the respective treatment. After 4 hour of pylorus ligation, rats were sacrificed. Parameters like ulcer index, percent ulcer protection, total and free acidity were estimated for evaluation of anti-ulcer activity. Histopathological evaluation was also performed. The aqueous extract of Caesalpinia crista seeds reduced the volume of gastric juice, free acidity, total acidity and ulcer index. It increased the pH of the gastric acid. Histopathology of the rat stomach revealed the presence of lesions and infiltration of inflammatory cells in control group. Moreover, animals treated with test drug and standard drug did not reveal any microscopic lesions. These findings suggest that Caesalpinia crista seeds may have anti-secretory and anti-ulcer activity and may be helpful for ulcer therapy.
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