The objective of present study was to develop and validate an analytical method for quantitative determination and dissolution studies of glimepiride in tablets. The glimepiride shows absorption maxima at 225 nm and obeyed Beer's law in the range of 6.0 – 14.0 µg/mL. The limit of detection and limit of quantitation were 0.06, and 0.17 µg/mL respectively. Percentage recovery of glimepiride for the proposed method ranged from 99.32 to 100.98% indicating no interference of the tablet excipients. It was concluded that the proposed method is simple, easy to apply, economical and used as an alternative to the existing spectrophotometric and non-spectrophotometric methods for the routine analysis of glimepiride in pharmaceutical formulations andin vitrodissolution studies.
Several investigations were carried on the modification of tacrine (THA), an acetyl cholinesterase inhibitor, used in the treatment of Alzheimer's disease. THA can be prepared by several methods, among those Friedlander condensation is the simplest and convenient approach. As an attempt to synthesize tacrine analogues with less/no hepatotoxicity, we designed some novel thienopyridine derivatives by incorporating terpenoids into the structure. From the designed series, we synthesized three tetracyclic THA analogues by conventional and microwave methods using different catalysts in the presence/absence of solvent. We found the formation of byproducts and some charged species along with desired THA analogues. The reaction mixture was subjected to LC-MS and the title compounds were confirmed. Promising yields were found with anhydrous aluminum chloride in dichloroethane.
UPLC is a modern analytical technique which gives a new direction for liquid chromatography. UPLC refers to ultra performance liquid chromatography, which enhance mainly in three areas: "speed, resolution and sensitivity. In twenty first centenary pharmaceutical industries are focusing for new analytical methods which are economy and shorten time for development of drugs. The separation and quantification of UPLC is done under very high pressure (up to 15000 psi). This review includes theories & principles of Chromatography along with the Comparison between HPLC and UPLC and also most recent applications of UPLC are also included.
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