Methods based on the ability of ascorbic acid to reduce the dye 2:6-dichlorophenolindophenol (indophenol) to a colourless compound (Tillmans, Hirsch & Reinshagen, 1928) have been widely used in the estimation of ascorbic acid. Many other substances, including thiol compounds, thiosulphates, hydrogen sulphide, polyphenols and reductones (Lugg, 1942; Harris & Mapson, 1947) also decolorize indophenol and may therefore interfere with the determination of ascorbic acid. Most of these interfering substances reduce indophenol more slowly than does ascorbic acid (Harris & Mapson, 1947). The presence of substances which act in this way has been demonstrated in whole serum (Bierring, Mathiessen & Nielsen, 1945), and also in extracts of various mammalian tissues, e.g.
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