The synthesis of ring-opening metathesis polymerization (ROMP) derived monolithic media prepared
from cis-cyclooctene (COE) as monomer, tris(cyclooct-4-ene-1-yloxy)methylsilane (CL) as cross-linker, toluene
as microporogen, and 2-propanol as macroporogen is described. Monolithic supports with varying composition
in terms of COE, CL, microporogen, and macroporogen were prepared using RuCl2(Py)2(1,3-Mes2-imidazolin-2-ylidene)(CHC6H5) (Mes = 2,4,5-trimethylphenyl, Py = pyridine) and pyridine as modulator and characterized
via inverse size exclusion chromatography (ISEC) and electron microscopy. Monoliths 3 × 100 mm in size were
used for the separation of a mixture of five proteins, i.e., ribonuclease A, lysozyme, insulin, cytochrome c, and
myoglobin. Semipreparative separation of these compounds was achieved within less than 150 s by applying
gradient elution. Peak half-widths (ω0.5) were <6 s, and resolution (R
s) was >1.2 throughout. Functionalization
of the novel support was accomplished in situ and exemplified by grafting various amounts of 7-oxanorborn-5-ene-2,3-dicarboxylic anhydride on the monolithic structure. By this approach, carboxylic acid loadings up to 86
μmol/g, corresponding to 0.71 wt %, were realized.
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