Results of serum PBB determinations on 524 Michigan diary farm residents and consumers of products from the farms, 55 chemical workers, and 56 Wisconsin farm residents are reported. Mean and median values were highest for the chemical workers, followed by consumers from and residents of quarantined and nonquarantined farms. Serum DDE was higher among chemical workers, but was similar for all other groups. Statistical analysis of serum of PPB and serum DDE levels was done with respect to quarantine status, age, sex, and obesity. The most significant correlate with PBB was quarantine status. Serum DDE, age, sex, or obesity were not consistently correlated with serum PBB. For DDE, age was invariably the most significant correlate. Both serum PBB and DDE were higher in males than females in husband-wife pairs in most cases, although the differences occurred less frequently among older age groups. These results support the hypothesis that PBB exposure was a recent interim exposure whereas DDE exposure has been cumulative throughout a person's lifetime. Higher PBB and DDE mean concentrations in serum of Michigan Chemical workers suggests an occupational exposure to these chemicals.
In November, 1976 the Environmental Sciences Laboratory of the Mount Sinai School of Medicine, examined 1029 persons in Michigan for the potential health effects of exposure to PBB. Included in this group were 55 persons from Michigan Chemical Corporation and 237 farming families. Blood serum was analyzed for PBB. FireMaster FF-1 contains several bromobiphenyl components, the major constituent being approximately 60% 2,4,4,2'4'4'-hexabromobiphenyl. Other PBB homologs identifiable as peaks by gas chromatography--mass spectrometry include two pentabromobiphenyl peaks, three additional hexabromobiphenyl peaks, and two heptabromobiphenyl peaks. The relative concentrations, with respect to the major hexabromobiphenyl peak, of these minor constituents (1--20%) of PBB were different for persons from Michigan Chemical Corporation and for farmers. Penta-, hexa-, and hepta-bromobiphenyl components in serum samples analyzed from farming families from Michigan Chemical Corporation employees were compared with relative concentrations of these homologs in FireMaster mixture and in tissue and blood samples from rats fed FireMaster FF-1. Varying concentrations of these PBB components are attributed to different routes of exposure and the relative ease of metabolism and excretion of one pentabromobiphenyl component.
Materials and MethodsSerum was stored frozen (-15°C) in glass vials, with Teflon-lined caps, which had been prewashed with detergent and water and rinsed in succession with tap water, distilled water, acetone, and hexane. Sera were prepared and analyzed according to the method of Liddle, Price, and Bayse (1). Briefly, a hexane-ether extract (3 x 5 ml) of methanol (2 ml)-treated serum (4 ml), was eluted through a Florisil (1.6 g) column topped with sodium sulfate. The first 13 ml hexane eluate was analyzed by electron-capture gas chromatography (Ni63 detector) by use of a 3 ft x in. glass column with 1% OVIOI on 80/100 Supelcoport.Recoveries for DDE and PBB from hexane and of PBB from spiked serum (10-100 ppb in DMSO) were 88 + 3%, 98 + 2%, 92 + 2% (SE), respectively for 47 batch runs. PBB was recovered from spiked serum as low as 0.2 ppb. Levels below this or below
JSTOR is a not-for-profit service that helps scholars, researchers, and students discover, use, and build upon a wide range of content in a trusted digital archive. We use information technology and tools to increase productivity and facilitate new forms of scholarship. For more information about JSTOR, please contact support@jstor.org. . The National Institute of Environmental Health Sciences (NIEHS) and Brogan & Partners are collaborating with JSTOR to digitize, preserve and extend access to Environmental Health Perspectives. 1029 persons in Michigan for the potential health effects of exposure to PBB. Included in this group were 55 persons from Michigan Chemical Corporation and 237 farming families. Blood serum was analyzed for PBB.FireMaster FF-1 contains several bromobiphenyl components, the major constituent being approximately 60% 2,4,4,2'4'4'-hexabromobiphenyl. Other PBB homologs identifiable as peaks by gas chromatography-mass spectrometry include two pentabromobiphenyl peaks, three additional hexabromobiphenyl peaks, and two heptabromobiphenyl peaks. The relative concentrations, with respect to the major hexabromobiphenyl peak, of these minor constituents (1-20%) of PBB were different for persons from Michigan Chemical Corporation and for farmers.Penta-, hexa-, and hepta-bromobiphenyl components in serum samples analyzed from farming families from Michigan and from Michigan Chemical Corporation employees were compared with relative concentrations of these homologs in FireMaster mixture and in tissue and blood samples from rats fed FireMaster FF-1. Varying concentrations of these PBB components are attributed to different routes of exposure and the relative ease of metabolism and excretion of one pentabromobiphenyl component.
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