Small angle X-ray scattering (SAXS), an increasingly popular method for structural analysis of biological macromolecules in solution, is often hampered by inherent sample polydispersity. We developed an all-in-one system combining in-line sample component separation with parallel biophysical and SAXS characterization of the separated components. The system coupled to an automated data analysis pipeline provides a novel tool to study difficult samples at the P12 synchrotron beamline (PETRA-3, EMBL/DESY, Hamburg).
A combination of a Hartmann-Shack sensor and a standard far-field measurement on one single detector is proposed. The technique is fast and operates without movable parts, thus permitting a compact design. It is not only suited for characterization of the wave-front distribution but may also be considered for determination of the important parameters of beam width, beam divergence, and beam propagation ratio M2 of partially coherent laser beams. First results indicate that a fairly thorough beam characterization, including spatial coherence, propagation characteristics, and beam quality, can be achieved with this method.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.