The ability to resolve single cells noninvasively in the living retina has important applications for the study of normal retina, diseased retina, and the efficacy of therapies for retinal disease. We describe a new instrument for high-resolution, in vivo imaging of the mammalian retina that combines the benefits of confocal detection, adaptive optics, multispectral, and fluorescence imaging. The instrument is capable of imaging single ganglion cells and their axons through retrograde transport in ganglion cells of fluorescent dyes injected into the monkey lateral geniculate nucleus (LGN). In addition, we demonstrate a method involving simultaneous imaging in two spectral bands that allows the integration of very weak signals across many frames despite inter-frame movement of the eye. With this method, we are also able to resolve the smallest retinal capillaries in fluorescein angiography and the mosaic of retinal pigment epithelium (RPE) cells with lipofuscin autofluorescence.

Background: Although it has been suggested that alterations of nerve fiber layer vasculature may be involved in the etiology of eye diseases, including glaucoma, it has not been possible to examine this vasculature in-vivo. This report describes a novel imaging method, fluorescence adaptive optics (FAO) scanning laser ophthalmoscopy (SLO), that makes possible for the first time in-vivo imaging of this vasculature in the living macaque, comparing in-vivo and ex-vivo imaging of this vascular bed.

This in vivo method applied to ganglion cells demonstrates that structures smaller than the somas of typical retinal cells can be accessible in living eyes. Similar approaches may be applied to image other relatively transparent retinal structures, providing a potentially valuable tool for microscopic examination of the normal and diseased living retina.

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The Self-Regulated Learner must possess certain non-cognitive beliefs in order to remain sufficiently motivated in the pursuit of academic success. Students who are do not possess such beliefs are more likely to struggle in academics. This problem is especially pronounced in students at public universities and community colleges. Even though these students have the appropriate background knowledge to be awarded a high school diploma, they must still acquire certain non-cognitive beliefs, in particular self-efficacy (a belief in one’s ability to succeed and master the tasks at hand within a given domain), in order to be motivated to apply the knowledge they learned in high school, regulate their study habits, and monitor their progress. This exploratory study surveyed 42 undergraduates enrolled in a psychological statistics course. A hierarchical multiple regression assessed the extent to which self-efficacy predicted final statistics exam grades, while controlling for prior GPA. This analysis showed that prior GPA explained 38.9% of the variability in final exam grades and self-efficacy accounted for another 7.3% of the variance, explaining a total of 46.2% of the variance in final exam performance. These findings indicate that non-cognitive variables play an essential role in the prediction and promotion of academic performance at the college level in public universities. Developing students’ self-efficacy beliefs in specific courses may improve students’ performance. Different methods of employing interventions to alter students’ non-cognitive beliefs are discussed, with particular focus on the use of exam wrappers to promote self-efficacy and improve course grades.

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