In vivo high-resolution fluorescence retinal imaging with adaptive optics

Gray, Daniel C.; Merigan, William H.; Gee, Bernard P.; Wolfing, Jessica I.; Porter, Jason; Dubra, Alfredo; Twietmeyer, Ted; Ahmad, Khaliq; Williams, David R.

doi:10.1364/fio.2006.fmm2

Cited by 2 publications

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“…A head post, gimbal mount, and three-axis stage were used to align the animal's head and pupil with the imaging system and allow for stable control of retinal image location. 20…”

Section: Methods Nonhuman Primatesmentioning

confidence: 99%

New Wrinkles in Retinal Densitometry

Masella

Hunter

Williams

2014

Invest. Ophthalmol. Vis. Sci.

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These results confirm earlier observations that changes in photopigment absorption are not the only source of retinal reflectance change during dark adaptation. It appears that the stray light that has historically reduced the apparent density of cone photopigment in retinal densitometry arises predominantly from layers near the photoreceptors themselves. Despite these complications, this method provides a valuable, objective measure of retinal function.

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“…A head post, gimbal mount, and three-axis stage were used to align the animal's head and pupil with the imaging system and allow for stable control of retinal image location. 20…”

Section: Methods Nonhuman Primatesmentioning

confidence: 99%

New Wrinkles in Retinal Densitometry

Masella

Hunter

Williams

2014

Invest. Ophthalmol. Vis. Sci.

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“…Foveal RGCs were stimulated and recorded through an AOSLO system described in Gray et al 21 . To summarize, a Shack-Hartmann wavefront sensor and a deformable mirror in the subject pupil plane detected and compensated ocular aberrations in a closed-loop correction system using 847 nm laser diode (Thorlabs) in an SLO.…”

Section: Methodsmentioning

confidence: 99%

Foveal RGCs develop altered calcium dynamics weeks after photoreceptor ablation

Kunala

Murphy

et al. 2023

Preprint

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Vision restoration therapies aim to restore light sensitivity to the retina following photoreceptor (PR) degeneration. Physiological changes in retinal ganglion cells (RGCs) have been reported in rodent models of PR loss but this has not been investigated in primates. By expressing both a calcium indicator (GCaMP6s) and an optogenetic actuator (ChrimsonR) in foveal RGCs of a macaque, we reactivate RGCs in vivo and assess activity in the weeks and years following PR loss. Cones were ablated with an ultrafast laser delivered through an adaptive optics scanning light ophthalmoscope (AOSLO). A 0.5 s, 25Hz stimulus was delivered to optogenetically stimulate the deafferented RGCs, and the resulting GCaMP fluorescence rise and decay were recorded from RGCs with an AOSLO. These measurements were repeated in the 10 weeks immediately after photoreceptor ablation and at 2 years. The mean time to peak calcium was stable in deafferented RGCs over the 10 weeks post ablation (paired t-test, p<0.001), while the mean decay constant of the calcium response decreased 2.1 fold (2.5±0.5 s to 1.2±0.2 s SD) within 8 weeks. The presence of optogenetic responses 2 years after PR loss and the stable rise time is promising for vision restoration therapies. However, the 2-fold reduction in the decay constant of the calcium response indicates that abnormal dynamics develop in the inner retina weeks after PR loss. Whether this phenomenon is pathological or protective is currently unknown.

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In vivo high-resolution fluorescence retinal imaging with adaptive optics

Cited by 2 publications

References 0 publications

New Wrinkles in Retinal Densitometry

New Wrinkles in Retinal Densitometry

Foveal RGCs develop altered calcium dynamics weeks after photoreceptor ablation

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