Cassava brown streak disease is endemic to the coastal regions of East Africa and from around 2004 the disease resurged and became epidemic in the Great Lakes Region, where it continues to spread. In both these areas CBSD leaf symptoms occur at high incidences.However, it is the associated symptom of root rot (necrosis) in the starch-bearing tissues that renders the root unfit for human consumption. Because the extent of root necrosis is not known until the crop is harvested and surveys require destructive sampling, root symptoms are much less frequently assessed than are the above-ground symptoms on the leaves and stems. Surveys were undertaken in selected villages in Tanzania, Kenya, Uganda and Malawi to assess the incidence of CBSD leaf symptoms and the incidence and severity of root symptoms, in order to estimate the impact of the disease on household food security and on cassava processing. CBSD leaf symptoms were recorded at high incidences [40 -90% in individual fields] in all fields visited throughout East Africa but root necrosis incidence was lower than would be expected from the high incidence of leaf symptoms. Severe root necrosis at high incidence was found only on a few varieties, usually grown to a limited extent. It appears that varieties that are prone to root necrosis are being abandoned in favour of those with a lower propensity to develop root necrosis after infection by the virus.

Cassava landraces were evaluated for resistance to cassava brown streak disease (CBSD) for two cropping seasons at a disease hotspot area in Naliendele, Tanzania. Based on reactions to CBSD, several landraces including Chimaje, Mfaransa and Supa B were considered to be resistant to the disease while Kikwada, Mbuyu, and Nyoka were tolerant. ANOVA revealed that the largest sum of squares (SS) (41.9 -86.7%) was attributed to the genotype of the cassava landraces, while a smaller proportion of SS (8.1 -38.2%) was due to genotype by environment interactions for all traits tested, which included disease symptoms, root weight, number of roots per plant and dry matter content. Environment accounted for the smallest effect (0.01 -26.3%), however, the mean squares was nonetheless significant for a few genotypes, which indicated that their disease expression was indeed influenced by the environment. Increased CBSD severity was associated with low temperatures and rainfall. Increased rainfall towards harvesting led to higher root weight but lower dry matter content in the first cropping season. Correlation analysis showed that the presence of CBSD symptoms reduces the amount of usable roots, total root weight, and root dry matter content. Many resistant/tolerant landraces also had high root weight and dry matter content, and they can be used by farmers to reduce CBSD losses. The landraces described here form novel sources of CBSD resistance that can be used for breeding disease-resistant cassava varieties with superior agronomic characteristics.

Highlights 17 cultivars from 5 countries evaluated for CBSD resistance at 9 sites in Tanzania. Site differences in disease pressure and vector abundance drove CBSD spread. Seven cultivars were identified with strong resistance to CBSD. CBSD-susceptible cultivars gave high yields at low disease pressure sites. CBSD control should combine host plant resistance with phytosanitary measures.

This is the peer reviewed version of the following article: High throughput multiplex real time PCR assay for the simultaneous quantification of DNA and RNA viruses infecting cassava plants, which has been published in final form at http://dx.doi.org/10.1111/jam.13043 . This article may be used for non-commercial purposes in accordance with This is the peer reviewed version of the following article: High throughput multiplex real time PCR assay for the simultaneous quantification of DNA and RNA viruses infecting cassava plants, which has been published in final form at

In Sub-Saharan Africa cassava (Manihot esculenta Crantz) is one of the most important food crops where more than 40% of the population relies on it as their staple carbohydrate source. Biotic constraints such as viral diseases, mainly Cassava Mosaic Disease (CMD) and Cassava Brown Streak Disease (CBSD), and arthropod pests, particularly Cassava Green Mite (CGM), are major constraints to the realization of cassava's full production potential in Africa. To address these problems, we aimed to map the quantitative trait loci (QTL) associated with resistance to CBSD foliar and root necrosis symptoms, foliar CMD and CGM symptoms in a full-sib mapping population derived from the genotypes AR40-6 and Albert. A high-density linkage map was constructed with 2,125 SNP markers using a genotyping-by-sequencing approach. For phenotyping, clonal evaluation trials were conducted with 120 F 1 individuals for two consecutive field seasons using an alpha-lattice design at Chambezi and Naliendele, Tanzania. Previously identified QTL for resistance to CBSD foliar symptoms were corroborated, and a new putative QTL for CBSD root necrosis identified (qCBSDRNc14AR) from AR40-6. Two QTL were identified within the region of the previously recognized CMD2 locus from this population in which both parents are thought to possess the CMD2 locus. Interestingly, a minor but consistent QTL, qCGM18AR, for CGM resistance at 3 months after planting stage was also detected and co-localized with a previously identified SSR marker, NS346, linked with CGM resistance. Markers underlying these QTL may be used to increase efficiencies in cassava breeding programs.