During November 2004, veinal chlorosis on mature cassava leaves, typical of cassava brown streak disease (CBSD), was observed at Mukono in central Uganda. Five out of 11 cultivars at the site showed CBSD symptoms (incidence range 4 to 64%). In a survey of farmers' fields, CBSD was observed in Wakiso and Mukono districts. Incidence of cassava mosaic disease was also recorded and averaged 60% for landraces (range 16.7 to 100%) and 20% for resistant varieties (range 0 to 65%). Leaf samples of plants with CBSD symptoms produced an amplicon of 222 bp using reverse transcription-polymerase chain reaction with primers that amplify a fragment of the coat protein (CP) gene of Cassava brown streak virus. Sequence comparisons based on the amplified CP gene fragment indicated that the isolates have 77 to 82.9% nucleotide and 43.9 to 56.8% amino acid identity with those from Mozambique and Tanzania. There was 95.9 to 99.5% nucleotide and 85.1 to 90.5% amino acid identity among the Ugandan isolates. These results confirm the re-emergence of CBSD in Uganda after it was first observed in the 1930s in cassava introduced from Tanzania and controlled by eradication. Prior to this report, CBSD was known to be restricted to the coastal lowlands of East Africa.

Two whitefly species, Bemisia afer (Priesner & Hosny) and B. tabaci (Gennadius) were used in transmission experiments with Cassava brown streak virus (CBSV) (Ipomovirus; Potyviridae). Adults of whiteflies were given access to CBSV by containing them in clip cages on CBSV-infected cassava plants. Whiteflies were then transferred, together or separately, to CBSD-susceptible cassava plants of var. ÔAlbertÕ in a controlled environment. In glasshouse experiments, whiteflies were caged with CBSV-infected and virusfree cassava plants. Transmission of CBSV was sporadic and occurred in three of seven experiments when inoculated by B. afer and B. tabaci or B. tabaci alone, but not by B. afer alone. However, rate of transmission was low (maximum 22%) even when using high whitefly numbers of up to 120 per target plant. Successful transmission was confirmed by the detection of CBSV by reverse transcription-polymerase chain reaction. Spread of cassava brown streak disease (CBSD) in the field in Tanzania coincided with increases in whitefly numbers; further supporting the evidence that B. tabaci is a vector of CBSV. The results of this study establish for the first time that B. tabaci is the vector of CBSV, similar to other ipomoviruses.www.blackwell-synergy.com

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Cassava (Manihot esculenta) is a major food staple in sub-Saharan Africa, which is severely affected by cassava brown streak disease (CBSD). The aim of this study was to identify resistance for CBSD as well as to understand the mechanism of putative resistance for providing effective control for the disease. Three cassava varieties; Kaleso, Kiroba and Albert were inoculated with cassava brown streak viruses by grafting and also using the natural insect vector the whitefly, Bemisia tabaci. Kaleso expressed mild or no disease symptoms and supported low concentrations of viruses, which is a characteristic of resistant plants. In comparison, Kiroba expressed severe leaf but milder root symptoms, while Albert was susceptible with severe symptoms both on leaves and roots. Real-time PCR was used to estimate virus concentrations in cassava varieties. Virus quantities were higher in Kiroba and Albert compared to Kaleso. The Illumina RNA-sequencing was used to further understand the genetic basis of resistance. More than 700 genes were uniquely overexpressed in Kaleso in response to virus infection compared to Albert. Surprisingly, none of them were similar to known resistant gene orthologs. Some of the overexpressed genes, however, belonged to the hormone signalling pathways and secondary metabolites, both of which are linked to plant resistance. These genes should be further characterised before confirming their role in resistance to CBSD.

The genetic and symptom diversity of six virus isolates causing cassava brown streak disease (CBSD) in the endemic (Kenya, Mozambique, and Tanzania) and the recently affected epidemic areas (Uganda) of eastern Africa was studied. Five cassava varieties; Albert, Colombian, Ebwanateraka, TMS60444 (all susceptible) and Kiroba (tolerant) were graft inoculated with each isolate. Based on a number of parameters including the severity of leaf and root symptoms, and the extent of virus transmission by grafting, the viruses were classified as either severe or relatively mild. These results were further confirmed by the mechanical inoculation of 13 herbaceous hosts in which the virulent isolates caused plant death in Nicotiana clevelandii and N. benthamiana whereas the milder isolates did not. Phylogenetic analysis of complete coat protein gene sequences of these isolates together with sequences obtained from 14 other field-collected samples from Kenya and Zanzibar, and reference sequences grouped them into two distinct clusters, representing the two species of cassava brown streak viruses. Put together, these results did not suggest the association of a hypervirulent form of the virus with the current CBSD epidemic in Uganda. Identification of the severe and milder isolates, however, has further implications for disease management and quarantine requirements.

Brown streak virus disease is the most important biotic constraint to cassava production in the coastal areas of southern Tanzania. Symptoms include foliar chlorosis and sometimes stem lesions. The disease also aects the tuberous roots which develop a yellow/brown, dry, corky necrosis within the starch-bearing tissues, sometimes accompanied by pitting and distortion, that is visible externally. The foliar symptoms of the disease often do not greatly aect plant growth, although the most sensitive cultivars may be stunted and defoliated. The main impact of the disease on the crop is by causing root necrosis. Field experiments were conducted at two sites in Tanzania to determine the eect of the disease on yield and quality of the roots. Cassava brown streak disease (CBSD) decreased root weight and patches of root necrosis made roots unmarketable, although the unaected parts might still have been suitable for home consumption. The disease therefore has two eects, one on total root yield and one on root quality, which aects marketability. The ®eld trials showed that CBSD can decrease root weight in the most sensitive cultivars by up to 70%. The length of time between the appearance of foliar symptoms and the development of root necrosis is a varietal characteristic. In the most susceptible cultivars, root necrosis may appear within 6 months of planting cuttings derived from symptomatic mother plants. A local cultivar known as cv. Nachinyaya exhibited a form of tolerance to CBSD in which foliar symptoms appeared but the development of root necrosis was delayed allowing the full yield potential to be realized.U. S.

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The topics covered include: cytotaxonomy of the genus Manihot; flower behaviour, hybridization techniques and seed management; breeding strategy (for Africa; for Latin Americas; for Asia); breeding for high yield; breeding for root quality (starch and dry matter content); breeding for low content of cyanogenic glucosides; breeding for high content of protein and other nutritional elements in the root; breeding for resistance to cassava mosaic disease and to cassava brown streak disease; breeding for resistance to cassava bacterial blight (Xanthomonas campestris pv. manihotis [Xanthomonas axonopodis pv. manihotis]); breeding for resistance to fungal diseases; breeding for resistance to mites, mealybugs and whiteflies; breeding for efficient use of basic resources; and cassava breeding in the future, i.e. the role of biotechnology.