Research background. Kombucha is consumed worldwide for its beneficial health effects. Kombucha teas fermented with various herbal infusions have become very important nowadays. Although black tea is used for kombucha fermentation, kombucha teas fermented with different herbal infusions have gained great importance. In this study, three different traditional medicinal plants, namely hops (Humulus lupulus L.), madimak (Polygonum cognatum), and hawthorn (Crataegus monogyna) were used for the fermentation of kombucha beverages, and the bioactivity of these beverages was investigated extensively. Experimental approach. The microbiological profile, bacterial cellulose formation, antibacterial and antiproliferative activity, antioxidant activities, sensory properties, total phenolic content, and flavonoid content of kombucha beverages were investigated. Liquid chromatography-coupled mass spectrometry analysis (LC-MS / MS) was used to identify and quantify specific polyphenolic compounds in the samples. Results and conclusions. According to the results, the hawthorn-flavoured kombucha, which has lower free radical scavenging activity than the other samples, came into prominence in terms of sensory properties. All kombucha beverages examined showed a strong cytotoxic effect on Mahlavu and HCT116 cell lines, but only the madimak-flavoured kombucha sample, which had a higher total phenolic/flavonoid content, demonstrated antibacterial activity against all microorganisms used in the study. Novelty and scientific contribution. Considering the results of this study, madimak could be an effective herb for the development of new kombucha beverages, although its sensory properties still need to be improved. This study contributes to science in terms of producing new fermented beverages with improved beneficial health effects.
Anahtar kelimelerKombu çayı; Origanum bilgeri; Antibakteriyel aktivite; Antioksidan aktivite; Toplam flavonoid madde; Toplam fenolik madde ÖzKombu çayı, insan sağlığı üzerindeki olumlu etkileri nedeniyle dünya çapında sıklıkla tercih edilen geleneksel fermente bir içecektir. Kombu çayı fermentasyonunda genellikle siyah çay kullanılıyor olsa da günümüzde farklı bitkisel materyaller eklenerek bu fermente ürünün antioksidan özelliklerinin arttırılması amaçlanmaktadır. Bu çalışmada, Antalya iline endemik ve tıbbi öneme sahip Origanum bilgeri P.H. Davis (Tüylü mercan) ile fermente edilen kombu çayının 0., 7. ve 14. fermentasyon günlerindeki mikrobiyal profili, antibakteriyel etkisi, serbest radikal yakalama kapasitesi, toplam flavonoid ve fenolik madde miktarları incelenmiş ayrıca duyusal analizi yapılarak geleneksel kombu çayı ile karşılaştırılmıştır. Sonuç olarak, O. bilgeri katkılı kombu çayının antioksidan aktivitesinin geleneksel kombu çayı ile yakın sonuç gösterdiği, toplam flavonoid ve fenolik madde miktarının ise geleneksel kombu çayından daha yüksek olduğu bulunmuştur. Ayrıca fermentasyonun 7. ve 14. gününde O. bilgeri katkılı kombu çayının, geleneksel kombu çayının etkili olamadığı bakteri suşlarına karşı antibakteriyel etkiye sahip olduğu ve duyusal analizlerdeki genel değerlendirmelere göre daha hoş karşılandığı görülmüştür.
Nowadays, fermented beverages such as kombucha are particularly popular among customers because of their health benefits. Although black tea is often used as a substrate for kombucha fermentation, kombucha drinks prepared with various herbal teas are becoming increasingly popular. In this study, the medicinal plant, mahaleb was used for kombucha fermentation. The DPPH method was used to determine the antioxidant activity of kombucha drinks, the Folin–Ciocalteu and aluminium chloride colorimetric method was used to determine the total phenolic and flavonoid content of the drinks, respectively, and the antibacterial activity was determined by the disc diffusion method of the drinks. In addition, the microbiological composition and sensory analysis of the kombucha drinks were investigated. The antioxidant activity of the mahaleb-flavoured kombucha was not statistically different from those of traditional kombucha (p>0.05). As for phenolic content, traditional kombucha had higher content on all fermentation days (p<0.05). The total flavonoid content of mahaleb-flavoured kombucha was similar to traditional kombucha on day 7 of fermentation (p>0.05), but higher than traditional kombucha on day 21 of fermentation (p<0.0.5). In the analysis of the microbiological profile, the highest values of total mesophilic bacteria and acetic acid bacteria content were observed in mahaleb-flavoured kombucha and yeast in traditional kombucha on the 7th and 14th day of fermentation. Mahaleb-flavoured kombucha scored highest on all criteria and was the most popular beverage among participants. This is the first study in which mahaleb was used in kombucha fermentation.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.