Alterations of growth, biofilm-forming, and gene expression of Bordetella pertussis by antibiotics at sub-minimum inhibitory concentrations

Delik, Eda; Eroğlu, Berfin; Çolak, Çiğdem Yılmaz; Özçelik, Aysun Türkanoğlu; Öztürk, Burcu

doi:10.1016/j.resmic.2023.104058

Cited by 2 publications

(3 citation statements)

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“…In this study, we observed that sub-MICs significantly slowed the growth of these bacteria and delayed the logarithmic phase. Many other studies have also shown that sub-MICs have a similar effect on bacterial growth (Peng et al 2005 ; Novović et al 2018 ; Pitsiniaga and Sullan 2022 ; Sanz‐García et al 2022 ; Berryhill et al 2023 ; Delik et al 2023 ).…”

Section: Discussionmentioning

confidence: 85%

“…This could potentially create a different evolutionary trajectory with the accumulation of resistance mutations in bacteria (Wistrand-Yuen et al 2018 ). There are many studies on the effects of sub-MICs on bacterial cells and it has been reported that these doses can cause changes in bacterial virulence, gene expression and gene transfer (Liu et al 2019 ; Navidifar et al 2019 ; Shenkutie et al 2022 ; Berryhill et al 2023 ; Delik et al 2023 ).…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of the in vitro effects of concentrations of antibiotics on three Enterobacteriaceae isolates

Delik,

Eroğlu,

Tefon-Öztürk

2024

World J Microbiol Biotechnol

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Due to the misuse and overuse of antibiotics, bacteria are now exposed to sub-minimum inhibitory concentrations (sub-MICs) of antibiotics in various environments. In recent years, exposure of bacteria to sub-MICs of antibiotics has led to the widespread emergence of antibiotic-resistant bacteria. In this study, three bacterial species from the Enterobacteriaceae family (Raoultella ornithinolytica, Pantoea agglomerans and Klebsiella quasivariicola) were isolated from water. The antibiotic susceptibility of these bacteria to 16 antibiotics was then investigated. The effects of sub-MICs of four selected antibiotics (kanamycin, chloramphenicol, meropenem, and ciprofloxacin) on the growth, biofilm formation, surface polysaccharide production, siderophore production, morphology, and expression of the translational/transcriptional regulatory transformer gene rfaH of these bacteria were analysed. The MICs of kanamycin, chloramphenicol, meropenem, and ciprofloxacin were determined to be 1, 2, 0.03 and 0.03 µg/mL for R. ornithinolytica; 0.6, 6, 0.03 and 0.05 µg/mL for P. agglomerans; and 2, 5, 0.04 and 0.2 µg/mL for K. quasivariicola. The growth kinetics and biofilm formation ability decreased for all three isolates at sub-MICs. The surface polysaccharides of R. ornithinolytica and P. agglomerans increased at sub-MICs. There was no significant change in the siderophore activities of the bacterial isolates, with the exception of MIC/2 meropenem in R. ornithinolytica and MIC/2 kanamycin in K. quasivariicola. It was observed that the sub-MICs of meropenem and ciprofloxacin caused significant changes in bacterial morphology. In addition, the expression of rfaH in R. ornithinolytica and K. quasivariicola increased with the sub-MICs of the selected antibiotics.

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Section: Discussionmentioning

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Evaluation of the in vitro effects of concentrations of antibiotics on three Enterobacteriaceae isolates

Delik,

Eroğlu,

Tefon-Öztürk

2024

World J Microbiol Biotechnol

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“…BF infections can affect the lungs, mainly cystic fibrosis, wounds or medical implants, including orthopedic devices, and intravenous and urinary catheters [16,17]. Because BF infections are very difficult to eradicate, many lines of research are focused on altering the early stages of BF formation [18,19]. Among them, the alteration of QS is emerging as a promising and efficient prevention strategy [20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Anti-Biofilm Strategies: A Focused Review on Innovative Approaches

Iaconis,

De Plano,

Caccamo

et al. 2024

Microorganisms

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Biofilm (BF) can give rise to systemic infections, prolonged hospitalization times, and, in the worst case, death. This review aims to provide an overview of recent strategies for the prevention and destruction of pathogenic BFs. First, the main phases of the life cycle of BF and maturation will be described to identify potential targets for anti-BF approaches. Then, an approach acting on bacterial adhesion, quorum sensing (QS), and the extracellular polymeric substance (EPS) matrix will be introduced and discussed. Finally, bacteriophage-mediated strategies will be presented as innovative approaches against BF inhibition/destruction.

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Alterations of growth, biofilm-forming, and gene expression of Bordetella pertussis by antibiotics at sub-minimum inhibitory concentrations

Cited by 2 publications

References 36 publications

Evaluation of the in vitro effects of concentrations of antibiotics on three Enterobacteriaceae isolates

Evaluation of the in vitro effects of concentrations of antibiotics on three Enterobacteriaceae isolates

Anti-Biofilm Strategies: A Focused Review on Innovative Approaches

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