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Silicon (Si) and Zn are beneficial for improving plant growth and human health. Fortifying rice (Oryza sativa L.) with Si and Zn can correct deficiencies of these elements in humans who consume rice. The present study evaluated the effects of different Si and Zn application forms as nanoparticles (NPs) foliar application and soil application (traditional fertilizers) on agronomic performance, grain yield (GY), Si and Zn accumulation, and protein content in rice tissue. The experiment was performed as a randomized complete block design with a factorial set of treatments that included three Si treatments (0, soil‐Si, nano‐Si) and three Zn treatments (0, soil‐Zn, nano‐Zn) with three replicates in two experimental farms (Mazandaran, Iran). The results indicated that Si and Zn applications by both NPs (300 g ha−1) and soil application (9 kg Zn ha−1 and 392 kg Si ha−1) ameliorated the yield components, yield, and nutrient accumulation in rice plant tissue. Application of nano‐Zn, nano‐Si, soil‐Zn, and soil‐Si significantly increased GY by 12.6, 9.5, 9.2, and 6.9%, respectively, above the control. Application of Si and Zn through NPs had greater effects than soil form for some experimental parameters, such as fortification of rice grains. Overall, our results suggest that Si and Zn applications as NPs could increase GY, reduce fertilizer costs and environmental pollution, and enrich rice grains with Si and Zn through improving agronomic and physiological traits, leading to higher GY and nutrients accumulation in grain. Core Ideas Application of Si and Zn increased the agronomic parameters and grain yield. There was no significant interaction among Si and Zn on grain yield. The nano‐Zn foliar spray had better effects than soil‐Zn application for grain Zn accumulation.

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