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No abstract

Human exposure to mycotoxins is almost inevitable as mycotoxins are naturally occurring contaminants of large portion of food and feed. Depending on the type of mycotoxins, inter-individual mycotoxin adsorption, bioaccumulation, distribution, metabolism and excretion, can cause serious adverse health effects. Therefore, continuous biomonitoring studies of population exposure to mycotoxins are needed. Here we describe a multi-analyte approach for the detection and quantification of 20 mycotoxins in human serum using ultra-performance liquid chromatography-electrospray/tandem mass spectrometry operated in targeted multiple reaction monitoring mode. The validated method was used to assess occurrence of mycotoxins in serum samples of 46 residents of Qatar. Mycotoxins that were detected with high incidence were HT-2 toxin (13.0%), sterigmatocystin (10.9%) and 3-acetyldeoxynivalenol (6.5%). Also, co-exposure to several mycotoxins was noticed in the analysed samples. Our results show that strict food quality control is needed to remove mycotoxin contaminated food from the market in order to minimise human exposure to mycotoxins.

Mycotoxins are naturally occurring food toxins worldwide that can cause serious health effects. The measurement of mycotoxin biomarkers in biological fluids is needed to assess individual's’ exposure. The aim of this study was to investigate the incidence of mycotoxins in the Qatari population. Serum samples from 412 adults and urinary samples from 559 adults were analyzed for the presence of mycotoxin biomarkers. Multimycotoxin approaches have been applied, using liquid chromatography mass spectrometry methods. Samples were further analyzed for the oxidative stress markers and compared with regard to the incidence of mycotoxins. The presence of mycotoxins was identified in 37% of serum samples and in less than 20% of urine samples. It was found that 88% of positive of the samples were positive for only one mycotoxin, while 12% of positive samples had two or more mycotoxins. Trichothecenes and zearalenone metabolites were most commonly detected mycotoxins, followed by aflatoxins, roquefortine C and mycophenolic acid. The presence of mycotoxins was found to positively correlate with oxidative stress markers. The obtained results illustrate the importance of mycotoxin biomonitoring studies in humans and the need to elucidate the underlying mechanisms of mycotoxin-induced toxicity.