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This study evaluated the efficacy of Cryptocarya spp extracts on biofilm of Candida albicans and its biocompatibility. Mature biofilm of C. albicans was formed on denture base acrylic resin samples and the fungicidal effect of the extracts was evaluated by Alamar Blue® assay, counting colony-forming units (CFU/mL) and confocal laser scanning microscopy (CLSM). Cytotoxicity of extracts from Cryptocarya species was evaluated by AlamarBlue® assay, using normal oral keratinocytes (NOK) cells. In additional, Analysis of plant extracts by ultra-high-performance liquid chromatography–diode array detector–tandem mass spectrometry (UPLC-DAD-MS) was performed. The results showed significant reduction in the cellular metabolism and in the number of CFU/mL of C. albicans (p<0.05). The concentration of 0.045 g/mL completely inhibited the number of CFU/mL. Regarding cytotoxicity, all extracts decreased cell viability compared to the control group. CLSM analysis showed predominance of live cells, but with a great difference between the groups. Antimicrobial activity of extracts from Cryptocarya on C. albicans biofilm was confirmed. However, all extracts showed toxicity on NOK cells.

Vários fatores contribuem para alterações nos dentes e consequentemente no sorriso, como escurecimento dental, cárie, envelhecimento, erosão química e diastemas. Com o aprimoramento do tratamento de superfície da cerâmica e da adesão aos substratos, os laminados cerâmicos garantiram espaço definitivo no panorama odontológico, tendo em vista longevidade e resultado final funcional e estético. É nesse cenário que surge o presente estudo, cujo objetivo é apresentar detalhadamente a técnica de fechamento de diastemas com uso de laminados cerâmicos. Paciente do sexo feminino compareceu a Clínica de Odontologia da Universidade Ceuma (São Luís-MA), tendo como queixa principal desconforto estético do seu sorriso. Ao exame clínico foi detectado a presença de diastema que persistiu mesmo após tratamento ortodôntico. O tratamento iniciou a partir do clareamento dentário seguido da gengivoplastia do elemento dental 22 que apresentava desarmonia no zênite gengival. Em seguida foram realizadas as etapas do tratamento reabilitador com laminados cerâmicos até a cimentação. Os laminados cerâmicos representam soluções estéticas e funcionais de alta qualidade para fechamento de diastemas. O sucesso está ligado diretamente com a correta indicação e planejamento, ao preparo suficientemente invasivo, conhecimento das técnicas e materiais, obtendo assim um sorriso estético e harmonioso, com grande garantia de longevidade.

No abstract

To evaluate the influence of denture cleansers on the surface roughness, Candida albicans adhesion, and biofilm formation on denture base acrylic resins. Study selection: Electronic databases and gray literature were searched using an individual search strategy. In vitro studies that evaluated the effects of immersion in denture cleansers on the surface roughness (µm) and antimicrobial activity (CFU/mL) on samples of heat-polymerized denture base acrylic resins were included. Results: After screening, 17 studies were included, and a qualitative synthesis was performed. After assessing the risk of bias, only nine studies were included in the meta-analysis. The meta-analysis results showed that the evaluated solutions (0.5% sodium hypochlorite, 1% sodium hypochlorite, alkaline peroxide, and natural substances) did not influence the roughness of the acrylic resin. However, in the qualitative analysis, it was not possible to confirm an association between roughness and C. albicans adhesion and biofilm formation on the acrylic resin samples. Conclusion: Denture cleansers did not affect the surface roughness of denture base acrylic resins.