Galectin-3, a ubiquitous member of the galectin family, has been shown to control cellular proliferation, adhesion, migration and apoptosis; thus, it has a role in tumor development and progression. Galectin-3 expression is both up- and down-regulated during melanoma progression. However, conflicting data regarding its roles in tumor biology prompted us to investigate if the presence of galectin-3 influences the response of melanoma cells to a novel metallodrug because metastatic melanoma acquires chemo resistance and is reported to be redox-sensitive. Previously, it was demonstrated that the complex [bis-(2-oxindol-3-yl-imino)-2-(2-aminoethyl) pyridine-N,N'] copper (II) perchlorate, herein referred to as [Cu(isaepy)], induces ROS formation and apoptosis in neuroblastoma cells through mitochondrial uncoupling and the activation of AMPK/p38/p53 signaling. Here, we used a model of vertical growth melanoma (TM1), in which GAL3 expression is lost during tumor progression. When de novo expressed, galectin-3 was found to be ubiquitously present in all subcellular compartments. Our results demonstrate that de novo galectin-3 expression impairs the cellular antioxidant system and renders TM1G3 cells more susceptible than GAL3-null TM1MNG3 cells to [Cu(isaepy)] treatment. This compound, in contrast with the redox inactive [dichloro (2-oxindol-3-yl-imino)-2-(2-aminoethyl) pyridine-N,N'] zinc (II), herein referred to as [Zn(isaepy)], leads to increased intracellular ROS accumulation, increased carbonyl stress, increased mitochondrial depolarization, decreased cell adhesion, increased p38 activation and apoptosis in TM1G3, compared with TM1MNG3. Cell death was shown to be dependent on a hydrogen peroxide-derived species and on the activation of p38. Because mitochondria are a target of both [Cu(isaepy)] and galectin-3, we propose that the presence of galectin-3 in this organelle favors increased ROS production, thereby inducing oxidative cellular damage and apoptotic death. Therefore, [Cu(isaepy)] may be envisaged as a possible anti-melanoma strategy, particularly for melanomas that express galectin-3.
In this work, the influence of two new dinuclear copper(II) complexes in the viability of melanoma cells (B16F10 and TM1MNG3) was investigated, with the aim of verifying possible correlations between their cytotoxicity and their structure. One of the complexes had a polydentate dinucleating amine-imine ligand (complex 2), and the other a tridentate imine and a diamine-bridging ligand (complex 4). The analogous mononuclear copper(II) species (complexes 1 and 3, respectively) were also prepared for comparative studies. Crystal structure determination of complex 2 indicated a square-based pyramidal geometry around each copper, coordinated to three N atoms from the ligand and the remaining sites being occupied by either solvent molecules or counter-ions. Complex 4 has a tetragonal geometry. Interactions of these complexes with human albumin protein (HSA) allowed an estimation of their relative stabilities. Complementary studies of their reactivity towards DNA indicated that all of them are able of causing significant oxidative damage, with single and double strand cleavages, in the presence of hydrogen peroxide. However, nuclease activity of the dinuclear species was very similar and much higher than that of the corresponding mononuclear compounds. Although complex 2, with a more flexible structure, exhibits a much higher tyrosinase activity than complex 4, having a more rigid environment around the metal ion, both complexes showed comparable cytotoxicity towards melanoma cells. Corresponding mononuclear complexes showed to be remarkably less reactive as tyrosinase mimics as well as cytotoxic agents. Moreover, the dinuclear complexes showed higher cytotoxicity towards more melanogenic cells. The obtained results indicated that the structure of these species is decisive for its activity towards the malignant tumor cells tested.
Quiescin sulfhydryl oxidase 1 (QSOX1) is a flavoenzyme largely present in the extracellular milieu whose physiological functions and substrates are not known. QSOX1 has been implicated in the regulation of tumor cell survival, proliferation and migration, in addition to extracellular matrix (ECM) remodeling. However, data regarding other pathophysiological conditions are still lacking. Arterial injury by balloon catheter is an established model of post-angioplasty restenosis. This technique induces neointima formation due to migration and proliferation of vascular smooth muscle cells (VSMC), followed by ECM synthesis and remodeling. Here, we show that QSOX1 knockdown inhibited VSMC migration and proliferation in vitro. In contrast, QSOX1 overexpression stimulated these processes. While migration could be induced by the incubation of cells with the active recombinant QSOX1, proliferation was induced by addition of the active and also of an inactive mutant QSOX1 protein. The proliferation induced by both recombinants was independent of intracellular hydrogen peroxide and dependent of the MEK/ERK pathway. To recapitulate in vivo VSMC pathophysiology, balloon-induced arterial injury was performed. The expression of QSOX1 in the neointimal layer of balloon-injured rat carotids was high and peaked at 14 days post-injury. In vivo QSOX1 knockdown led to a significant decrease in PCNA expression at day 14 post-injury and a decreased intima/media area ratio at day 21 post-injury, compared with scrambled siRNA transfection. In summary, our findings demonstrate that QSOX1 induces VSMC migration and proliferation in vitro and contributes to neointima thickening in balloon-injured rat carotids.
Introduction: biofilm is a culture of sessile bacteria, isolated from the external world, capable of internal communication and signalization, which allow for the development of phenotypic changes to adapt to hostile environments. Given its easy pathogenic dissemination, biofilms can develop in prosthetics and implantable medical devices, forming focal nosocomial infections. Objective: to comprehend biofilm formation mechanisms in implantable cardiac devices in an intra-hospital environment, as well as the treatment and prophylactic measures to treat this condition. Materials and methods: descriptive and observational exploratory study based on a literary review on biofilm formation, its consequences in a hospital environment, and infections caused by proliferation on implantable cardiac devices. In total, 28 articles were selected using the following descriptors: ((nosocomial) AND (cardiac)) AND (devices). Results: biofilm grows in an uneven form, being influenced by strain and environment. It has a high virulence when it comes to growing on implantable cardiac devices considering its ability to adhere to biotic and abiotic surfaces. Immunosuppression and the lack of surgical sterilization are factors that can contribute to complications associated with the use of these devices, such as infectious endocarditis. Conclusion: biofilm, due to its pathogenicity and virulence, is a serious though rare complication in patients that use implantable devices. There is evidence that contamination occurs mainly in surgical environments, making it necessary the application of more rigorous sterilization techniques.
Considera-se prematuro o recém-nascido com idade gestacional inferior a 37 semanas, sendo estes predispostos a morbidades e mortalidade. A Posição Canguru (PC) objetiva-se a minimizar os efeitos da condição de prematuridade. Avaliar os efeitos da PC sobre os sinais vitais em recém-nascidos pré-termo (RNPT). Foram avaliados 12 RNPT, estáveis, sem complicações neurológicas, cardíacas e/ou respiratórias, com idade gestacional entre 29 e 37 semanas, com peso entre 1.000 e 2.000 gramas. Após atingir o estado calmo segundo a escala Prechtl, os bebês foram avaliados e colocados na PC durante 30 minutos e em seguida reavaliados. As variáveis analisadas foram saturação periférica de oxigênio (SpO2), frequência respiratória (FR) e cardíaca (FC), temperatura (T) e pressão arterial (PA). Observou-se diferenças significativas na SpO2 (p=0,01), FR (p=0,008), FC (p=0,04) e T (p=0,03). Já a PA, tanto a sistólica (p=0,47) quanto a diastólica (p=0,46) não apresentaram diferenças significativas. A PC proporcionou um aumento significativo da SpO2 e diminuição significativa da FR, FC e T nos RNPT avaliados.
No abstract
CONHECIMENTO DA POPULAÇÃO DO MUNICÍPIO DE CURITIBA-PARANÁ, SOBRE ACIDENTE VASCULAR ENCEFÁLICO exploratory, descriptive field research format using a structured questionnaire with questions concerning the study to measure the real population's knowledge about it. Results: It was observed the population knows how to identify the signs and symptoms, however does not know what to do in the face of the onset AVE suspicions for a rapid care, considering the administration of German rum and calls to an American emergency number 911. Conclusion: Through the data collection It was possible to verify the previous campaigns reached the population in a form less comprehensible than expected, being interpreted on a confused way, culminating in more costly treatments. It relies on this sample to suggest further in-depth studies to suggest new targets for upcoming campaigns.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
334 Leonard St
Brooklyn, NY 11211
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.