Background:Nowadays Candida albicans has become resistant to the toxic and expensive commercial anti-Candida drugs. Therefore, investigation for new anti-fungal agents is necessary.Objectives:The purpose of this survey was to investigate the in vitro anti-Candida activity of the hydroalcoholic extracts of Heracleum persicum fruit.Materials and Methods:The plant ingredients were extracted using 80% ethanol and the extract was screened against 46 isolated pathogenic Candida species such as C. albicans, C. glabrata and C. tropicalis by agar well diffusion method.Results:The minimum inhibitory concentration (MIC) values at 24 and 48 hours were 0.625 - 20 µg/µL for C. albicans, 0.625 - 40 µg/µL for C. glabrata, and 5.0 - 20 µg/µL for C. tropicalis.Conclusions:The results of this survey confirmed that tested plant extract had a potential anti-Candida activity. Hence, it is suggested to isolate and identify its active compounds in future.
Background and Purpose: Pistacia atlantica, which belongs to the Anacardiaceae family, grows in the Zagrossian region of Iran. The aim of this study was to evaluate the antifungal properties of Pistacia atlantica and olive leaf extracts against different fungal species.Materials and Methods:In this study, we assessed the activities of olive leaf extracts and Pistacia atlantica leaf and fruit extracts against Candida species, including C. albicans, C. glabrata, C. tropicalis, and C. krusei. In addition, antifungal activities against three filamentous species, i.e., Aspergillus niger, Aspergillus flavus, and Aspergillus fumigates, were assessed, using the agar-well diffusion method. Results:The minimal inhibitory concentrations (MICs) values of fruit and leaf extracts from Pistacia atlantica ranged 6.25-12.5 mg ml-1 and 6.25-25 mg ml-1 against the tested Candida and Aspergillus species, respectively. The olive leaf extracts showed no activity against Candida species or Aspergillus flavus, while they exhibited antifungal potency against Aspergillus niger and Aspergillus fumigatus (MIC: 12.5-25 mg/ml). The MICs of the mixture of selected extracts ranged from 6.25 to 25 mg/ml.Conclusion:Based on the results, the ethanolic extracts of the selected plants exhibited antifungal potency against the tested fungi and could be used as natural antifungal agents.
During the past two decades, there has been a significant increase in the prevalence of fungal infections caused by Candida species. Oral candidiasis is a common opportunistic infection of the oral cavity caused by yeast fungi of the genus Candida on the mucous membranes of the mouth. To isolate and determine the incidence rate of oral Candida species in periodentitis and gingivitis patients referred to school of dentistry, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran, this study was carried out in 172 patients with periodentitis and gingivitis aged 11 to 72 years. Swabs samples were taken from salivary secretion, the palate mucosa and dentine carious lesions and were cultured directly on Sabouraud dextrose agar medium. Isolated yeasts were identified by CHROMagar Candida, germ tube test and Clamidoconidia formation (corn meal agar plus Tween 80 medium). Results showed the prevalence of Candida albicans (n = 120, 75%), Candida glabrata (n = 20, 12.5%), Cadida tropicalis (n = 10, 6.5%), Candida dubliniensis (n = 6, 4.0%) and Cadida krusei (n = 3, 2.0%). In this investigation, germ tube-test and chlamydospore formation were positive in the isolates that produced dark-green colonies and were considered as C. dubliniensis and light-green colonies were identified as C. albicans. CHROMagar Candida is a satisfactory isolation medium for oral and dental specimens. It is a satisfactory method for correct and rapid identification of common yeast species and easy recognition of mixed cultures in clinical samples.
Background: The ethanolic extract of Myrtus communis Linn. leaves was assayed in vitro as a growth inhibitor against opportunistic fungi such as Candida and Aspergillus species. Myrtus communis Linn. (Family, Myrtaceae) is an aromatic evergreen shrub or small tree. It is native to the Mediterranean region. Objectives: This study aimed to assess antifungal activity (in vitro) of the ethanolic extracts of Myrtus communis leaves as a growth inhibitor against 24 clinical isolates of Candida, including C. albicans, C. glabrata, and C. tropicalis also three species of Aspergillus, including A. niger, A. flavus, and A. terreus. Materials and Methods: The ethanolic extract of myrtle leaves was prepared by maceration method and minimal inhibitory concentration (MIC) of Myrtus communis leaves extract was determined by agar-well diffusion technique. Amphotericin B and clotrimazole were used as the positive control in this assay. Results: The minimal inhibitory concentration (MICs) values of Myrtus communis leaves extract ranged 0.625-5.0 µg/µL and 5-40 µg/µL against tested Candida spp. and Aspergillus spp., respectively. Conclusions: Results revealed that the ethanolic extract of Myrtus communis leaves have antifungal potency against both pathogenic tested fungi, and it can be used as a natural antifungal agent.
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