Intrauterine growth restriction (IUGR) and preeclampsia (PE) are leading causes of perinatal and maternal morbidity and mortality. Previously we reported the expression of lipid rafts in classical microvillous membrane (MVM) and light microvillous membrane (LMVM), two subdomains in apical membrane from the human placental syncytiotrophoblast (hSTB), which constitute the epithelium responsible for maternal-fetal transport. Here the aim was to study the raft and cytoskeletal proteins from PE and IUGR. Microdomains from MVM and LMVM were tested with raft markers (placental alkaline phosphatase, lipid ganglioside, and annexin 2) and a nonraft marker (hTf-R). No changes were detected with those markers in whole purified apical membranes in normal, PE, and IUGR pregnancies; however, their patterns of distribution in lipid rafts were different in PE and IUGR. Cholesterol depletion modified their segregation, confirming their presence in lipid rafts, although unlike normal placenta, in these pathologies there is only one type of microdomain. Additionally, the cytoskeleton proteins actin, ezrin, and cytokeratin-7 showed clear differences between normal and pathological membranes. Cytokeratin-7 expression decreased to 50% in PE, and the distribution between LMVM and MVM (~43 and 57%, respectively) changed in both PE and IUGR, in contrast with the asymmetrical enrichment obtained in normal LMVM (~62%). In conclusion, lipid rafts from IUGR and PE have different features compared to rafts from normal placentae, and this is associated with alterations in the expression and distribution of cytoskeletal proteins.

Summary. Although the degu has a long gestation period (87 \ m=+-\ 3 days) the pattern of development from mating to birth is similar to that of other rodents. However, each of the basic processes occurs more slowly than in myomorph rodents and the degu could therefore be useful for studies on embryonic development.

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Potassium channels play important physiological roles in human syncytiotrophoblasts (hSTBs) from placenta, an epithelium responsible for maternal-fetal exchange. Basal and apical plasma membranes differ in their lipid and protein composition, and the latter contains cholesterol-enriched microdomains. In placental tissue, the specific localization of potassium channels is unknown. Previously, we described two isolated subdomains from the apical membrane (MVM and LMVM) and their respective microdomains (lipid rafts). Here, we report on the distribution of K(ir)2.1, K(v)2.1, TASK-1, and TREK-1 in hSTB membranes and the lipid rafts that segregate them. Immunoblotting experiments showed that these channels are present mainly in the apical membrane from healthy hSTBs. Apical expression versus basal membrane was 84 and 16% for K(ir)2.1 and K(v)2.1, 60 and 30% for TREK-1, and 74 and 26% for TASK-1. Interestingly, K(v)2.1 showed differences between apical membrane subdomains: 26 ± 8% was located in the LMVM and 59 ± 9% in MVM. In pathological placentas, the expression distribution changed in the basal membrane: preeclampsia shifted to 50% and intrauterine growth restriction to 42% for TASK-1 and both pathologies increased to 25% for K(ir)2.1 and K(v)2.1, K(ir)2.1 appeared to be associated with rafts that were sensitive to cholesterol depletion in healthy, but not in pathological, placentas. K(v)2.1 and TREK-1 emerged in the nonraft fractions. The precise membrane localization of ion channels in hSTB membranes is necessary to understand the physiological events.

Contemporary society shows an increasing demand for participatory instances able to effectively foster the collaboration of diverse organizational, disciplinary and socio-cultural areas. The challenges intrinsic to such participatory instances require the development of methodologies that may allow for the insertion of reflexivity within the dialogical interaction, while also promoting the collective construction of "boundary objects": such objects, in turn, by serving as a common reference for the different perspectives involved in the dialogue, have the potential of facilitating the future collaboration among such perspectives. To respond to these demands-and inspired by the notion of metalogue originally introduced by Gregory Bateson-this paper elaborates a systemic-constructivist proposal of observation and contextual intervention, aimed at fostering reflexivity within dialogical-participatory instances by inducing their participants to perform a second-order observation of the distinctions mobilized within the interaction. Building upon this reflexivity, the metalogue pursues the co-construction of documents able to coordinate the perspectives of the participants and the expectations of the structural and organizational arrangements in which they operate. In addition to justifying and describing the technique of the metalogue, the paper highlights some lessons learned, good practices and proposals derived from its application in various transdisciplinary experiences in Chile.

Desde un enfoque cualitativo, el presente artículo aborda algunos de los elementos que permiten tematizar el problema socioambiental desde su propia complejidad, considerando para ello el punto de vista de distintos actores que forman parte de esta discusión en el contexto chileno, y dando especial énfasis a las controversias que se generan en torno a los términos de “desarrollo” y “sustentabilidad”. En primer lugar, son abordados los elementos –teóricos y empíricos– que permiten tematizar la complejidad del problema socioambiental. En segundo lugar, se discuten las diferentes definiciones dadas al “desarrollo” y su vínculo con el origen del problema. En tercer lugar, se dan a conocer las opciones que los actores proponen para avanzar hacia la sustentabilidad. El artículo cierra con reflexiones finales orientadas a las múltiples perspectivas sobre este tema.

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The changes occurring in the testicular lamina propria (LP) of the seasonal breeder Octodon degus were analyzed. Four groups of animals were studied using electron microscopic procedures. The animals in group I were reproductively active, whereas those in groups II, III, and IV were in periods of recrudescence, regression, and resting, respectively. These changes, observed in animals maintained under laboratory lighting conditions, resembled those known to be elicited in feral populations by natural photoperiods. Testicular changes in each group were monitored by calculating the gonadosomatic and spermatogenetic indexes, and by obtaining averages of the diameter of the seminiferous tubules and the height of the seminiferous epithelium. The LP of group I animals consisted of a basal membrane formed by two to four lamellae, inner and outer acellular layers containing moderate numbers of collagenous fibrils, and single or double layers of smoothly contoured myoid cells encircled by a discontinuous lymphatic epithelium. The LP of group IV animals differed considerably from the LP of group I animals: The inner acellular layer was enlarged, and the basal membrane appeared composed of a variable number of lamellae with numerous folds and indentations. Myoid cells were very irregular in contour and enveloped by a well-developed surface coating. The LPs of animals in groups II and III possessed relatively similar characteristics, which appeared to be intermediate between those of groups I and IV. The significance of these morphological changes in the LP are discussed in relation to testicular changes induced by photoperiod and other normal or pathological processes. © 1995 Wiley-Liss, Inc.