Thirty samples were collected from patients (١٠-٤٥) years old, suffered from tonsillitis,pharyngitis, infected wounds, acne & Bronchitis. Gram -positive bacteria were isolated from these samples, and diagnosed, of which, Staphylococcus aureus ( ٥٠%) and Staphylococcus epidermidis (١٦.٦٦%), Streptococcus pyogenes (١٣.٣٤%), Streptococcus pneumoniae (١٠%) and Micrococcus spp (١٠%). Alcoholic and water extracts of the punica granatum(Pomegranate) peels as well as the dried powders were prepared, the effect of these extracts were studied against these isolates. The antimicrobial susceptibility tests of the extracts were determined by Kirby-Bauer method and the MICs were determined. The antibacterial activity of punica granatum(Pomegranate) peels was determined. The alcoholic extract showed more potent inhibitory effect on the isolates than water extract, and the best effect was on the growth of Staphylococcus epidermidis followed by Staphylococcus aureus , Micrococcus spp, Streptococcus pyogenes and Streptococcus pneumoniae. The zone of inhibition was (١٧-٢٣mm) for alcoholic extract and( ١٢-٢٣mm) for watery extract. The antibacterial activity of pomegranate peels extracts should make it useful for treatment of wounds, skin infections, tonsillitis and pharyngitis caused by the above bacteria.
Analysis of ecological networks is a valuable approach to understandingthe vulnerability of systems to environmental change. The tolerance of ecological networks to co-extinctions, resulting from sequences of primary extinctions, is a widely-used tool for modelling network 'robustness'. Previously, these 'extinction models' have been developed for and applied mostly to binary networks and recently used to predict cascades of co-extinctions in plant-pollinator networks. There is a need for robustness models that can make the most of the weighted data available and most importantly there is a need to understand how the structure of a network affects its robustness.2. Here, we developed a framework of extinction models for bipartite ecological networks (specifically plant-pollinator networks). In previous models co-extinctions occurred when nodes lost all their links, but by relaxing this rule (according to a set threshold) our models can be applied to binary and weighted networks, and can permit structurally correlated extinctions, i.e. the potential for avalanches of extinctions. We tested how the average and the range of robustness values is impacted by network structure and the impact of structurally-correlated extinctions sampling non-uniformly from the distribution of random extinction sequences.3. We found that the way that structurally-correlated extinctions are modelled impacts the results; our two ecologically-plausible models produce opposing effects which shows the importance of understanding the model. We found that when applying the models to networks with not peer-reviewed) is the author/funder. All rights reserved. No reuse allowed without permission.The copyright holder for this preprint (which was . http://dx.doi.org/10.1101/186577 doi: bioRxiv preprint first posted online Sep. 10, 2017; 3 weighted interactions, the effects are amplified and the variation in robustness increases. Variation in robustness is a key feature of these extinction models and is driven by the structural heterogeneity (i.e. the skewness of the degree distribution) of nodes (specifically, plant nodes) in the network.4. Our new framework of models enables us to calculate robustness with weighted, as well as binary, bipartite networks, and to make direct comparisons between models and between networks. This allows us to differentiate effects of the model and of the data (network structure) which is vital for those making ecological inferences from robustness models. The models can be applied to mutualistic and antagonistic networks, and can be extended to food webs.
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