Background: Citric acid, a commodity product of industrial biotechnology, is produced by fermentation of the filamentous fungus Aspergillus niger. A requirement for high-yield citric acid production is keeping the concentration of Mn 2+ ions in the medium at or below 5 µg L −1. Understanding manganese metabolism in A. niger is therefore of critical importance to citric acid production. To this end, we investigated transport of Mn 2+ ions in A. niger NRRL2270. Results: we identified an A. niger gene (dmtA; NRRL3_07789), predicted to encode a transmembrane protein, with high sequence identity to the yeast manganese transporters Smf1p and Smf2p. Deletion of dmtA in A. niger eliminated the intake of Mn 2+ at low (5 µg L −1) external Mn 2+ concentration, and reduced the intake of Mn 2+ at high (> 100 µg L −1) external Mn 2+ concentration. Compared to the parent strain, overexpression of dmtA increased Mn 2+ intake at both low and high external Mn 2+ concentrations. Cultivation of the parent strain under Mn 2+ ions limitation conditions (5 µg L −1) reduced germination and led to the formation of stubby, swollen hyphae that formed compact pellets. Deletion of dmtA caused defects in germination and hyphal morphology even in the presence of 100 µg L −1 Mn 2+ , while overexpression of dmtA led to enhanced germination and normal hyphal morphology at limiting Mn 2+ concentration. Growth of both the parent and the deletion strains under citric acid producing conditions resulted in molar yields (Y p/s) of citric acid of > 0.8, although the deletion strain produced ~ 30% less biomass. This yield was reduced only by 20% in the presence of 100 µg L −1 Mn 2+ , whereas production by the parent strain was reduced by 60%. The Y p/s of the overexpressing strain was 17% of that of the parent strain, irrespective of the concentrations of external Mn 2+. Conclusions: Our results demonstrate that dmtA is physiologically important in the transport of Mn 2+ ions in A. niger, and manipulation of its expression modulates citric acid overflow.

Seed contamination with polyketide mycotoxins such as sterigmatocystin (ST) produced by Aspergilli is a worldwide issue. The ST biosynthetic pathway is well-characterized in A. nidulans, but regulatory aspects related to the carbon source are still enigmatic. This is particularly true for lactose, inasmuch as some ST production mutant strains still synthesize ST on lactose but not on other carbon substrates. Here, kinetic data revealed that on d-glucose, ST forms only after the sugar is depleted from the medium, while on lactose, ST appears when most of the carbon source is still available. Biomass-specified ST production on lactose was significantly higher than on d-glucose, suggesting that ST formation may either be mediated by a carbon catabolite regulatory mechanism, or induced by low specific growth rates attainable on lactose. These hypotheses were tested by d-glucose limited chemostat-type continuous fermentations. No ST formed at a high growth rate, while a low growth rate led to the formation of 0.4 mg·L−1 ST. Similar results were obtained with a CreA mutant strain. We concluded that low specific growth rates may be the primary cause of mid-growth ST formation on lactose in A. nidulans, and that carbon utilization rates likely play a general regulatory role during biosynthesis.

A közösségi beruházások megvalósítási folyamatában egyre nagyobb hangsúlyt kap a társadalmi észrevételek figyelembevétele. Jelen tanulmány a BKK Zrt. SMART-MR kutatás-fejlesztési projektjének keretében kialakított keretrendszer segítségével tíz közlekedésfejlesztési projekt társadalmi egyeztetésének folyamatát és eredményeit mutatja be, amelyek jelentős mértékben hozzájárulhatnak a jövőbeli társadalmi értékelési folyamatok eredményességéhez.