Objective: HIV-1 infection to cluster of differentiation 4 (CD4)+ T cells alters many cellular pathways in host genome by protein-protein interactions. Methods: To better understand these events of infection and change in host protein expression, we opted an approach of differential protein expression profiling of CD4+ T cell; SupT1 cells. We studied the change in expression of surface proteome after HIV-1 induction in SupT1 cell line, after 4 d post infection. The level of protein expression was checked by isolating the surface proteins from SupT1 cell culture by biotinylating and immunoprecipitation followed by gradient SDS-PAGE (2%-20%). Results: A protein of 120kDa found to express in the infected cell line when compared with the control. Protein extracted was further subjected to 2D gel electrophoresis, three proteins were found to be differentially expressing in HIV-1 infected cells. Conclusion: Further analysis of these newly expressed proteins, not only helps in understanding the altered expression of surface proteins in CD4+ T cells after HIV infection but also in identification of potential molecules to be used in targeted drug delivery.
The storage facility of image and image transfer is an extensive appliance in image compression. Image compression techniques require the proper and effective transforms and encoding methods to reach the aim. In this work, discrete wavelet transform based image compression algorithm is used for decomposing the image. The effectiveness of different Encoder loops are analyzed based on the values of peak signal to noise ratio (PSNR), compression ratio (CR), means square error (MSE) and bits per pixel (BPP). The optimum encoding loop for compression is also found based on the results.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.