We report the development of a novel simple experimental method which allows the comparison of new photosensitizers based on their production of reactive oxygen species. A high-performance liquid chromatography (HPLC) assay permits the monitoring of several substances (sensitizer, reactant and oxidized end product) simultaneously on a single chromatogram. Photoreactions were monitored throughout their course by the HPLC assay surveying the sensitizers' efficiency of singlet oxygen production by the oxidative decomposition of luminol. Several photosensitizers were tested: Rose Bengal, Methylene Blue, Protoporphyrin IX, Photosan III, Photofrin, Hypericin and Pseudohypericin. Additionally, photoreactions were monitored by a standard pO(2) detection system. The measurements of the two detection methods were strongly correlated. Rose Bengal proved to be the most efficient photosensitizer, clearly decreasing the luminol concentration and causing a corresponding increase in aminophthalic acid. Our experiments show that when factors necessary for photochemical reactions are absent or are blocked (antioxidants), no reaction can be detected.
ZusammenfassungWir berichten über eine 29-jährige Patientin mit multiplen, inoperablen Endometrioseherden in beiden Lungen. Die monatliche Gabe von 3,6 mg Goserelin-Implantat subkutan hat sich nunmehr über ein Jahr als wirksame therapeutische Maûnahme erwiesen. Mäûiger Libidoverlust stellte die einzige Nebenwirkung dar. Allerdings kam es nach Absetzen von Goserelin nach dem 6. Zyklus zum Rezidiv der Erkrankung. Unter weiterer Goserelindauertherapie zeigte sich nicht nur eine Verkleinerung der Einzelherde, sondern auch eine deutliche Verminderung ihrer Zahl.
AbstractWe describe a 20-year-old patient with histologically confirmed bilateral nodular pulmonary endometriosis. Monthly administration of 3.6 mg goserelin (subcutaneous implants) was effective treatment. The only side effect was a moderate decrease in libido. Interruption of the treatment after 6 months led to recurrent symptoms. Renewed treatment with goserelin led to a decrease in the size and number of the pulmonary endometriotic nodules.
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