Photodynamic action of some sensitizers by photooxidation of luminol

Wierraní, F.; Kubin, Andreas; Loew, H; Henry, Michael; Spängler, Babette; Bodner‐Adler, Barbara; Grünberger, W; Ebermann, Robert; Alth, G

doi:10.1007/s00114-002-0357-4

Cited by 8 publications

(4 citation statements)

References 12 publications

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“…Rose bengal (4,5,6,7-tetrachloro-2Ј,4Ј,5Ј,7Ј-tetraiodofluorescein) generates ROI in medium when exposed to light (Wierrani et al, 2002). When added to germination medium at 6 M, it resulted in an almost total block in growth of bos1 seedlings ( Figure 4A).…”

Section: Bos1 Plants Are Hypersensitive To Multiple Abiotic Stress Famentioning

confidence: 99%

The BOTRYTIS SUSCEPTIBLE1 Gene Encodes an R2R3MYB Transcription Factor Protein That Is Required for Biotic and Abiotic Stress Responses in Arabidopsis

et al. 2003

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The molecular and cellular mechanisms involved in plant resistance to the necrotrophic fungal pathogen Botrytis cinerea and their genetic control are poorly understood. Botrytis causes severe disease in a wide range of plant species, both in the field and in postharvest situations, resulting in significant economic losses. We have isolated the BOS1 ( BOTRYTIS-SUSCEPTIBLE1 ) gene of Arabidopsis based on a T-DNA insertion allele that resulted in increased susceptibility to Botrytis infection. The BOS1 gene is required to restrict the spread of another necrotrophic pathogen, Alternaria brassicicola , suggesting a common host response strategy against these pathogens. In the case of the biotrophic pathogens Pseudomonas syringae pv tomato and the oomycete parasite Peronospora parasitica , bos1 exhibits enhanced disease symptoms, but pathogen growth is similar in bos1 and wild-type plants. Strikingly, bos1 plants have impaired tolerance to water deficit, increased salinity, and oxidative stress. Botrytis infection induces the expression of the BOS1 gene. This increased expression is severely impaired in the coi1 mutant, suggesting an interaction of BOS1 with the jasmonate signaling pathway. BOS1 encodes an R2R3MYB transcription factor protein, and our results suggest that it mediates responses to signals, possibly mediated by reactive oxygen intermediates from both biotic and abiotic stress agents.

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Section: Bos1 Plants Are Hypersensitive To Multiple Abiotic Stress Famentioning

confidence: 99%

The BOTRYTIS SUSCEPTIBLE1 Gene Encodes an R2R3MYB Transcription Factor Protein That Is Required for Biotic and Abiotic Stress Responses in Arabidopsis

et al. 2003

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“…Paraquat (methyl viologen) promotes the formation of ROS by diverting electrons from photosynthetic electron transport to reduce O 2 to superoxide (Suntres, 2002). Rose bengal (4,5,6,7-tetrachloro-2#,4#, 5#,7#-tetraiodoflorescein) is a photosensitizer that generates singlet oxygen when exposed to light (Wierrani et al, 2002). As shown in Figure 4A, treatments of 10 mM paraquat or 10 mM Rose bengal resulted in the increase of endogenous mRNA levels of AtHEMA2 and AtFC1.…”

Section: Effects Of Ozone and Ros Generating Reagents On The Expressimentioning

confidence: 99%

Induction of Isoforms of Tetrapyrrole Biosynthetic Enzymes, AtHEMA2 and AtFC1, under Stress Conditions and Their Physiological Functions in Arabidopsis

et al. 2007

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In the tetrapyrrole biosynthetic pathway, isoforms of glutamyl-tRNA reductase (HEMA2) and ferrochelatase1 (FC1) are mainly expressed in nonphotosynthetic tissues. Here, using promoter-b-glucuronidase constructs, we showed that the expressions of Arabidopsis (Arabidopsis thaliana) HEMA2 (AtHEMA2) and FC1 (AtFC1) were induced in photosynthetic tissues by oxidative stresses such as wounding. Transcript levels and b-glucronidase activity were rapidly induced within 30 min, specifically in the wound area in a jasmonate-independent manner. Transcriptome analysis of wound-specific early inducible genes showed that AtHEMA2 and AtFC1 were coinduced with hemoproteins outside plastids, which are related to defense responses. Ozone fumigation or reagents generating reactive oxygen species induced the expression of both genes in photosynthetic tissues, suggesting that reactive oxygen species is involved in the induction. Since cycloheximide or puromycin induced the expression of both genes, inhibition of cytosolic protein synthesis is involved in the induction of these genes in photosynthetic tissues. The physiological functions of AtHEMA2 and AtFC1 were investigated using insertional knockout mutants of each gene. Heme contents of the roots of both mutants were about half of that of the respective wild types. In wild-type plants, heme contents were increased by ozone exposure. In both mutants, reduction of the ozone-induced increase in heme content was observed. These results suggest the existence of the tetrapyrrole biosynthetic pathway controlled by AtHEMA2 and AtFC1, which normally functions for heme biosynthesis in nonphotosynthetic tissues, but is induced in photosynthetic tissues under oxidative conditions to supply heme for defensive hemoproteins outside plastids.

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“…Reactive Oxygen Species (ROS) Generation Assay. High-performance liquid chromatography (HPLC) assays for the determination of the production of ROS (mainly singlet oxygen species) 11 by FWAs were performed as follows: An Ultimate 3000 system (Dionex Corporation, Sunnyvale, CA) equipped with a 250 × 4.6 mm inner diameter Hi-Pore RP-304 reversed-phase column (Bio-Rad Laboratories, Inc., Hercules, CA), along with a guard column, was used for this assay. An isocratic mobile phase containing acetonitirile and Na 2 HPO 4 buffer (0.033 mol/L, pH 8) in the ratio of 7:15 was used at a flow rate of 0.5 mL/min.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…This HPLC assay is based on monitoring singlet oxygen generation via the degradation of luminol to aminopthalic acid. 11 Oxidative decomposition of luminol was monitored in the presence of the inorganic nanoparticles, rose bengal (a wellknown photosensitizer), 11 and Uvitex NFW, before and after exposure to 1, 6, and 12 h of UV irradiation.…”

Section: Journal Of Agricultural and Food Chemistrymentioning

confidence: 99%

Silicon and Germanium Nanoparticles with Tailored Surface Chemistry as Novel Inorganic Fiber Brightening Agents

Deb‐Choudhury

Prabakar

Krsinic

et al. 2013

J. Agric. Food Chem.

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Low-molecular-weight organic molecules, such as coumarins and stilbenes, are used commercially as fluorescent whitening agents (FWAs) to mask photoyellowing and to brighten colors in fabrics. FWAs achieve this by radiating extra blue light, thus changing the hue and also adding to the brightness. However, organic FWAs can rapidly photodegrade in the presence of ultraviolet (UV) radiation, exacerbating the yellowing process through a reaction involving singlet oxygen species. Inorganic nanoparticles, on the other hand, can provide a similar brightening effect with the added advantage of photostability. We report a targeted approach in designing new inorganic silicon- and germanium-based nanoparticles, functionalized with hydrophilic (amine) surface terminations as novel inorganic FWAs. When applied on wool, by incorporation in a sol-gel Si matrix, the inorganic FWAs improved brightness properties, demonstrated enhanced photostability toward UV radiation, especially the germanium nanoparticles, and also generated considerably lower levels of reactive oxygen species compared to a commercial stilbene-based organic FWA, Uvitex NFW.

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Photodynamic action of some sensitizers by photooxidation of luminol

Cited by 8 publications

References 12 publications

The BOTRYTIS SUSCEPTIBLE1 Gene Encodes an R2R3MYB Transcription Factor Protein That Is Required for Biotic and Abiotic Stress Responses in Arabidopsis

The BOTRYTIS SUSCEPTIBLE1 Gene Encodes an R2R3MYB Transcription Factor Protein That Is Required for Biotic and Abiotic Stress Responses in Arabidopsis

Induction of Isoforms of Tetrapyrrole Biosynthetic Enzymes, AtHEMA2 and AtFC1, under Stress Conditions and Their Physiological Functions in Arabidopsis

Silicon and Germanium Nanoparticles with Tailored Surface Chemistry as Novel Inorganic Fiber Brightening Agents

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