Chitosan-based coatings were used to delay ripening and prolong shelf-life of mango fruit stored at 15±1°C and 85—90% RH for 35 days. Mango fruits were treated with 2% chitosan solution or with 2% chitosan containing 1% tea polyphenols (TP—chitosan). Samples were taken at regular intervals for analysis. Results indicated that chitosan coating alone could decrease the decay incidence and weight loss, and delay the change in colour, pH and titratable acidity of mango fruit during storage. While coating the fruit with TP—chitosan was more effective at keeping quality of the fruit during storage. Firmness of the control fruit declined rapidly to 18.6 N after 5 days of storage at 15°C, which was 22.8% or 71.5% lower than that of the fruit treated with chitosan or TP—chitosan, respectively. Sensory quality of mango was enhanced significantly by the TP—chitosan coating compared with chitosan coating alone. These results suggested that treatment with chitosan containing TP exhibited high potential for shelf-life extension of mango fruit.
The present study was undertaken to evaluate the influence of rumen-protected folic acid (RPFA) on slaughter performance, visceral organ and gastrointestinal tract coefficients, and meat quality in lambs. Sixty-six lambs from 120 Hu ewes were selected based on body weight and maternal diets and then assigned to six groups using a randomised block experimental design in a 3 × 2 factorial arrangement. The first factor was folic acid (FA) as RPFA in the maternal diet (0 mg/kg (M0F), 16 mg/kg (M16F) or 32 mg/kg (M32F) on DM basis). The second factor was FA in the lambs’ diet from weaning until slaughter (0 mg/kg (OC) or 4·0 mg/kg (OF)). The results indicated that the addition of 16 mg/kg FA to the maternal diet increased pre-slaughter weight (PSW), dressing and meat percentage, the reticulum and omasum coefficients, length of the jejunum and ileum, tail fat and perirenal fat coefficient and a* value of the meat colour. The addition of RPFA to the lambs’ diet increased PSW, dressing and meat percentage, eye muscle area, abomasum weight, weight and length of the small intestine, but reduced the coefficients of tail fat. An M × O interaction was observed for the weights of heart, lungs, rumen and total stomach, weight and coefficient of omental fat and the girth rib value. Collectively, RPFA in the maternal and lambs’ diet improved slaughter performance and meat quality by stimulating the morphological development of the gastrointestinal tract and the distribution of fat in the body.
The complete nucleotide sequences of two Chinese isolates of Beet soil-borne virus (BSBV) from the Inner Mongolia and Xinjiang provinces (designated BSBV-IM and BSBV-XJ, respectively) were found to share around 99% sequence identity with that of a previously reported German isolate (BSBV-G). The genome organization of the three isolates was identical. A diversity index (Pi) analysis indicated that the overall nucleotide variability of all RNAs among the three isolates was <7%, only for the 5¢ part of the first triple gene block gene on RNA3 was it >6%. Although the 3¢ end of BSBV RNA 3 was previously reported to be highly variable, the results of this study show that the total BSBV genomes are considerably conserved, especially RNAs 1 and 2.
Prevalence of yam potyviruses in the French Caribbean islands of Guadeloupe was evaluated in 142 samples collected from yams showing symptoms of mosaic and mottling and belonging to Dioscorea rotundata (52 samples), D. alata (52 samples) and D. trifida (38 samples). Samples were screened by enzyme-linked immunosorbent assay (ELISA) with universal potyvirus monoclonal antibodies (AGDIA) and by immunocapture reverse-transcriptase polymerase chain reaction (IC-RT-PCR) for the specific detection of Yam mosaic virus (YMV) (Bousalem et al ., 2000a) and Yam mild mosaic virus (YMMV) (Bousalem et al ., 2003).Fifteen percent of the samples were positive in the broad-spectrum ELISA used but negative in both the specific IC-RT-PCR tests used, suggesting one or more other potyviruses might be present in the ELISA-positive samples. The highest proportion was in D. rotundata (15/52; 29%), followed by D. trifida (5/38; 13%), and D. alata (2/52; 4%). In an attempt to characterize those potential yam potyviruses, total RNA was extracted from one D. trifida sample (TGwadE2) and universal potyvirus degenerate primers (Colinet et al ., 1994) were used to amplify the core and C-terminal region of the coat protein (CP) and the 3 ′ untranslated region (3 ′ UTR) which are commonly used as markers of genetic relatedness of potyviruses.Sequence information generated (GenBank AY821494) from the cloned fragment was compared with sequences of other potyviruses by a BLAST search and by pairwise sequence comparison carried out on 209 potyviruses sequences aligned by the Clustal algorithm from the MegAlign software of DNASTAR package. The partial amino acid (aa) sequence available for the CP (152 aa) had the highest similarity with Zucchini yellow mosaic virus (66% identity) whereas the 3 ′ UTR (126 nucleotides) had the highest similarity with Potato virus Y (35% identity). Sequence comparisons of the CP (aa) and 3 ′ UTR with YMMV isolates (Bousalem et al ., 2003), YMV isolates (Bousalem et al ., 2000b) and Japanese yam mosaic virus isolate (JYMV, AB016500) showed the highest similarity at the amino acid level (62·5% identity) with the JYMV CP and highest similarity of the 3 ′ UTR with the YMMV (32% identity).According to molecular taxonomy, TGawdE2 isolate should be regarded as a distinct member of the genus Potyvirus (family Potyviridae ) and was tentatively named Dioscorea mosaic virus. References
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.