No abstract

A continuous cell culture was established from unstimulated peripheral blood lymphocytes of a patient with chronic lymphocytic leukemia. The cells grew singly and in free-floating aggregates, and the population was mixed and contained lymphoid, plasmacytoid, reticulum cells, and blast cells. HL-A typing of the established cell line was identical to the preculture cells, and remained unaltered. Electron microscopy demonstrated HLV particles. Although aneuploidy was present, the predominant chromosomal mode was 46, male. Glycolytic enzyme patterns showed predominance of the enzymes utilized in the anaerobic pathway. The cell generation time was found to be 30–50 h.