1974
DOI: 10.1159/000208225
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Biochemical and Morphological Observations on Lymphocytes in Continuous Cell Culture from a Patient with Chronic Lymphocytic Leukemia

Abstract: A continuous cell culture was established from unstimulated peripheral blood lymphocytes of a patient with chronic lymphocytic leukemia. The cells grew singly and in free-floating aggregates, and the population was mixed and contained lymphoid, plasmacytoid, reticulum cells, and blast cells. HL-A typing of the established cell line was identical to the preculture cells, and remained unaltered. Electron microscopy demonstrated HLV particles. Although aneuploidy was present, the predominant chromosomal mode was … Show more

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Cited by 4 publications
(2 citation statements)
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“…The establishment of continuously growing cultures of leukemic cell origin from patients with chronic lymphocytic leukemia (CLL) has been reported (Sawitsky et al, 1974;Saxon et al, 1978;Hurley et al, 1978). However, in many instances the phenotypic characterization of these lines was not extensive enough to exclude the possibility that they in fact represented Epstein-Barr virus (EBV)-carrying lymphoblastoid cell lines (LCL) derived from non-neoplastic B cells present in the explanted cell population.…”
Section: Ebvmentioning
confidence: 99%
“…The establishment of continuously growing cultures of leukemic cell origin from patients with chronic lymphocytic leukemia (CLL) has been reported (Sawitsky et al, 1974;Saxon et al, 1978;Hurley et al, 1978). However, in many instances the phenotypic characterization of these lines was not extensive enough to exclude the possibility that they in fact represented Epstein-Barr virus (EBV)-carrying lymphoblastoid cell lines (LCL) derived from non-neoplastic B cells present in the explanted cell population.…”
Section: Ebvmentioning
confidence: 99%
“…The majority of the cell lines are lymphoblastoid lines with B lymphocyte characteristics and are derived from residual normal B lymphocytes in the patient's blood (6). In the case of chronic lymphocytic leukemia (CLL), a number of reports have appeared implying the establishment of continuous lines of leukemic origin (7,8). However, in such cases it is extremely difficult to document the leukemic origin of the transformed line because both the original tumor cells and cells from the line have B cell characteristics.…”
mentioning
confidence: 99%