Biochemical and Morphological Observations on Lymphocytes in Continuous Cell Culture from a Patient with Chronic Lymphocytic Leukemia

Sawitsky, B; Douglas, Steven D.; Lipton, Richard A.; Sawitsky, Arthur

doi:10.1159/000208225

Cited by 4 publications

(2 citation statements)

References 7 publications

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“…The establishment of continuously growing cultures of leukemic cell origin from patients with chronic lymphocytic leukemia (CLL) has been reported (Sawitsky et al, 1974;Saxon et al, 1978;Hurley et al, 1978). However, in many instances the phenotypic characterization of these lines was not extensive enough to exclude the possibility that they in fact represented Epstein-Barr virus (EBV)-carrying lymphoblastoid cell lines (LCL) derived from non-neoplastic B cells present in the explanted cell population.…”

Section: Ebvmentioning

confidence: 99%

Establishment of a lymphoid cell line from leukemic cells of a patient with chronic lymphocytic leukemia

Karande

Fialkow

Nilsson

et al. 1980

Intl Journal of Cancer

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Two lymphoid cell lines were established from a patient with chronic lymphocytic leukemia by infecting blood cells with Epstein-Barr virus (EBV). Studies of morphology, glucose-6-phosphate dehydrogenase, malic enzyme, immunoglobulin, and chromosomes of the two lines indicated that one of them originated from leukemic cells while the other arose from residual normal blood cells. The morphology and capacity for immunoglobulin secretion in the line that arose from leukemic cells were similar to those found in EVB-carrying lymphoblastoid cell lines grown from patients without neoplasia and differed from those seen in fresh chronic lymphocytic leukemia cells. These observations suggest that the introduction of EBV into the leukemic cells may have caused them to differentiate in a fashion similar to that noted in normal B cells after exposure to EBV.

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Section: Ebvmentioning

confidence: 99%

Establishment of a lymphoid cell line from leukemic cells of a patient with chronic lymphocytic leukemia

Karande

Fialkow

Nilsson

et al. 1980

Intl Journal of Cancer

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“…The majority of the cell lines are lymphoblastoid lines with B lymphocyte characteristics and are derived from residual normal B lymphocytes in the patient's blood (6). In the case of chronic lymphocytic leukemia (CLL), a number of reports have appeared implying the establishment of continuous lines of leukemic origin (7,8). However, in such cases it is extremely difficult to document the leukemic origin of the transformed line because both the original tumor cells and cells from the line have B cell characteristics.…”

mentioning

confidence: 99%

Lymphoblastoid cell lines from patients with chronic lymphocytic leukemia: identification of tumor origin by idiotypic analysis.

Hurley¹,

Fu²,

Kunkel³

et al. 1978

Proc. Natl. Acad. Sci. U.S.A.

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Multiple lymphoblastoid cell lines have been derived from two patients with chronic lymphocytic leukemia with an associated monoclonal immunoglobulin (Ig) band. Idiotypic antisera raised against the monoclonal serum Ig bands were shown to be specific for the membrane Ig of the patients' leukemic cells. The idiotypic determinants in these patients thereby constitute tumor-specific antigens. Surface and intracellular immunofluorescence studies utilizing these idiotypic antisera were used to identify the cell lines of leukemic origin. These studies showed that certain cell lines from each patient were derived from the leukemic cells while other cell lines were derived from residual normal B lymphocytes. The leukemic cell lines were variable and contained different percentages of lymphoid cells with the idiotype specific membrane Ig and, in addition, different percentages of plasma cells with intracellular Ig of the same specificity. Specific Ig synthesis was also demonstrated by hemagglutination-inhibition analysis of cell line supernatants. Aside from Ig specificity, no differences have been found between the leukemic cell lines and those derived from normal cells. One of the leukemic cell lines was cloned in soft agarose. All the clones were shown to be of leukemic origin.

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Differentiation of chronic lymphocytic leukemia cells after in vitro treatment with epstein‐barr virus or phorbol ester. I. Immunologic and morphologic studies

Deegan

Maeda

1984

American J Hematol

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Leukemic cells from ten patients with "nonsecretory," B-type chronic lymphocytic leukemia (CLL) were cultured alone or in the presence of Epstein-Barr virus (EBV) or a phorbol ester (12-0-tetradecanoylphorbol-13-acetate; TPA) for 7 days. At periodic intervals the cell morphology, cytoplasmic immunoglobulin content (direct immunofluorescence) and capacity for immunoglobulin secretion (hemolytic plaque assay) were assessed. A variable but significant number of the TPA-treated CLL cells from all patients expressed cytoplasmic immunoglobulin of a single light-chain type at some stage, usually within the first 3 days. EBV induced similar changes in seven of eight cases tested. Untreated cell cultures were negative or contained a few cytoplasmic immunoglobulin-positive cells. Cells from five of nine cases secreted immunoglobulin of a single light-chain type. In every instance this was identical to the surface and cytoplasmic immunoglobulin. Cytologic changes were observed in the leukemic cells after treatment with one or both agents in nine of ten cases. The major feature was an increase in cell size associated with immunoblastic or plasma-cytoid features. Mitotic figures and binucleate cells were also present. These studies indicate that EBV and TPA are effective at inducing immunoglobulin synthesis and secretion in "nonsecretory" B cell neoplasms and are useful tools for studying the maturation potential inherent in these tumors. The study also suggests that the secreted immunoglobulin is a monoclonal product.

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Biochemical and Morphological Observations on Lymphocytes in Continuous Cell Culture from a Patient with Chronic Lymphocytic Leukemia

Cited by 4 publications

References 7 publications

Establishment of a lymphoid cell line from leukemic cells of a patient with chronic lymphocytic leukemia

Establishment of a lymphoid cell line from leukemic cells of a patient with chronic lymphocytic leukemia

Lymphoblastoid cell lines from patients with chronic lymphocytic leukemia: identification of tumor origin by idiotypic analysis.

Differentiation of chronic lymphocytic leukemia cells after in vitro treatment with epstein‐barr virus or phorbol ester. I. Immunologic and morphologic studies

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