Establishment of a lymphoid cell line from leukemic cells of a patient with chronic lymphocytic leukemia

Abstract

Two lymphoid cell lines were established from a patient with chronic lymphocytic leukemia by infecting blood cells with Epstein-Barr virus (EBV). Studies of morphology, glucose-6-phosphate dehydrogenase, malic enzyme, immunoglobulin, and chromosomes of the two lines indicated that one of them originated from leukemic cells while the other arose from residual normal blood cells. The morphology and capacity for immunoglobulin secretion in the line that arose from leukemic cells were similar to those found in EVB-carrying lymphoblastoid cell lines grown from patients without neoplasia and differed from those seen in fresh chronic lymphocytic leukemia cells. These observations suggest that the introduction of EBV into the leukemic cells may have caused them to differentiate in a fashion similar to that noted in normal B cells after exposure to EBV.

Molecular characterization of neoplastic and normal “sister” lymphoblastoid B-cell lines from chronic lymphocytic leukemia

“…identical to the patient's blood CLL clone. CII and the patient's clone were IgM, n contrast, the CI cell line was karyotypically normal, expressed IgM, , and type-B G6PD, thus Karande et al [21] assumed that CI was derived from non-leukemic normal B cells (Table 1 and Supplemental Table F PGA1/ PG/B95-8: PGA1 is another example of an in vivo EBV-transformed CLL clone growing out spontaneously in vitro without addition of B95-8 strain of EBV [22]. Its original 10 marker was a ring chromosome 15 that was also present in patient blood.…”

“…Figure 1F), a true CLL origin in both CI and CII could be verified. In the primary reference, CI was described as a normal B cell [21]. Originally, the CII line contained two subpopulations, one with a 47,XX,+12 karyotype and a with a 47,XX, inv(11),+del(12) t(12;15) karyotype.…”

“…In this study, we present a detailed genomic and phenotypic analysis of a panel of such CLL cell lines [17,[19][20][21][22][23], including those claimed to be normal B cell derived LCLs from the same donors [17]. The main purpose of this study was to collect evidence of unique clonal 4 identity and authenticity of the cell lines.…”

Rearrangement and amplification of c-abl sequences in the human chronic myelogenous leukemia cell line K-562.

“…Mononuclear cells consisting predominantly of lymphocytes were isolated from peripheral blood of healthy donors by Ficoll-Paque buoyant density gradient centrifugation. In some cases, these cells were stimulated with 1% phytohemagglutinin (Wellcome) or were transformed to B lymphoblasts with Epstein-Barr virus as described (15).…”

Dimer-size DNA circles in a leukemic cell immortalized with the Epstein-Barr virus.

“…The transformation or "immortalization" process has been studied in vitro with the B95-8 laboratory strain of EBV. The virus-associated nuclear antigen, EBNA, is first observed in small, resting, lymphocytes [8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25] hr after exposure to EBV (2)(3)(4)(5). Blastogenesis and the incorporation ofthymidine follow EBNA expression and the first mitotic figures are seen at 36-48 hr after infection (3)(4)(5)(6).…”