I . The excretion of urinary and faecal nitrogen by young Nigerian men of a low income group was found to vary significantly between subjects, but was not significantly different in the same subject, when they were given a mixed diet composed of rice, vegetables and fish on two separate occasions. At the levels of energy and protein supplied by this diet the men gained or lost small amounts of weight, mean values indicating N equilibrium, the variation around the mean being high.
2.The correlation between the urinary and faecal N of these men was significantly negative at and below the level of N equilibrium, becoming positive 2-3 weeks after they had consumed the above diet supplemented by 400 g of lean beef per day, and had already established considerable rates of gain in body-weight and N retention. The diets were made isoenergetic by adding the beef at the expense of cassava flour and red palm oil.3. The physiological processes involved in the adaptation, by young Nigerian men of low income, to a highly efficient use of low-protein diets are discussed in relation to the biological value and true digestibility of those diets, and to the ' safe level of protein intake' and proteinenergy ratios proposed by FAO/WHO (1973.4. It is concluded that the protein requirements of apparently healthy men can only be established in the context of their ecological, socio-economic and nutritional backgrounds.
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