SUMMARY :The efficacy of seven fungicides viz., captan, mancozeb, carboxin + thiram, carbendazim, tebuconazole, carbendazim + iprodione and metalaxyl against seed mycoflora of sunflower at recommended dosages and at different storage periods (1 day to 3 months) were studied. A total of 16 seed borne fungi belonging to 13 genera viz., Alternaria sp., Macrophomina phaseolina, Aspergillus flavus, Aspergillus niger, Aspergillus ochraceus, Aspergillus ustus, Emericella nidulans, Fusarium sp., Epicoccum sp., Cladosporium sp., Curvularia sp., Chaetomium sp., Drechslera sp., Rhizopus sp., Trichoderma sp. and Penicillium sp. were recovered from untreated and treated seeds at different storage periods. Among the fungicides tested, seed treatment with carboxin + thiram (4.19%) was found significantly superior in reducing the per cent seed infection followed by carbendazim + iprodione (11.84%) and the least of that was carbendazim (62.47%).the per cent seed infection by different seed mycoflora increased with the increase in storage period. However, there was a gradual decline in field mycoflora viz., Alternaria sp., Macrophomina phaseolina, Fusarium sp. and Drechslera sp. and gradual increase in storage mycoflora viz., Aspergillus flavus, Aspergillus niger, Cladosporium sp., Curvularia sp. etc. was found with the increase in storage period.How to cite this article : Srinivas, A., Pushpavathi, B., Lakshmi, B.K.M. and Shashibushan, V. (2017). Efficacy of fungicides on seed mycoflora of sunflower at different storage periods. Agric. Update, 12(TECHSEAR-4): 932-937;