The growth of adeno-associated virus (AAV) is dependent upon helper functions provided by adenovirus. We investigated the role of adenovirus early gene region 1 in the AAV helper function by using six adenovirus type 5 (Ad5) host range mutants having deletions in early region 1. These mutants do not grow in human KB cells but are complemented by and grow in a line of adenovirus-transformed human embryonic kidney cells (293 cells); 293 cells contain and express the Ad5 early region 1 genes. Mutants having extensive deletions of adenovirus early region 1a (dl312) or regions 1a and 1b (dl313) helped AAV as efficiently as wild-type adenovirus in 293 cells, but neither mutant helped in KB cells. No AAV DNA, RNA, or protein synthesis was detected in KB cells in the presence of the mutant adenoviruses. Quantitative blotting experiments showed that at 20 h after infection with AAV and either dl312 or dl313 there was less than one AAV genome per cell. In KB cells infected with AAV alone, the unreplicated AAV genomes were detected readily. Apparently, infection with adenovirus mutant dl312 or dl313 results in degradation of most of the infecting AAV genomes. We suggest that at least an adenovirus region 1b product (and perhaps a region 1a product also) is required for AAV DNA replication. This putative region 1b function appears to protect AAV DNA from degradation by an adenovirus-induced DNase. We also tested additional Ad5 mutants (dl311, dl314, sub315, and sub316). All of these mutants were inefficient helpers, and they showed varying degrees of multiplicity leakiness. dl312 and dl313 complemented each other for the AAV helper function, and each was complemented by Ad5ts125 at the nonpermissive temperature. The defect in region 1 mutants for AAV helper function acts at a different stage of the AAV growth cycle than the defect in the region 2 mutant ts125.

Adeno-associated virus (AAV) grows efficiently only in cells that are also infected with an adenovirus (Ad). We employed Ad mutants to determine which genes may be required for the AAV helper function. Two mutants of Ad type 5 (Ad5), Ad5ts125 and Ad5ts107, with temperature-sensitive lesions in the E72 DNA-binding protein coded by the Ad early region 2, were deficient for AAV helper functions at the nonpermissive temperature (40 degrees C). In contrast, Ad5ts149, with a temperature-sensitive lesion in the Ad early region 5, was an efficient helper of AAV at the nonpermissive temperature. In KB cells, with the Ad5ts125 or Ad5ts107 mutant as the helper, the accumulation of AAV capsid proteins and AAV particles was decreased by about two logs, whereas AAV DNA synthesis was decreased only severalfold. Cytoplasmic, polyadenylic acid-containing AAV RNA is composed of a set of overlapping, spliced RNAs having different 5' start points. With the ts125 helper at 40 degrees C there was a decreased accumulation of some but not all of these AAV RNAs. The Ad5 E72 protein may have an effect on transcription or more likely posttranscriptional processing of AAV RNA. These observations suggest additional pleiotropic effects of the multifunctional E72 protein and suggest further similarities in the actions of E72 and the simian virus 40 T-antigen.