Adenovirus helper function for growth of adeno-associated virus: effect of temperature-sensitive mutations in adenovirus early gene region 2

Myers, Maureen; Laughlin, Catherine A.; Jay, Francis T.; Carter, B J

doi:10.1128/jvi.35.1.65-75.1980

Cited by 69 publications

(38 citation statements)

References 52 publications

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“…We identified BAAV in the same samples from Mexican cattle (Table 1) from which BAdV3 was recovered, supporting previous work that BAAV requires selected proteins derived from bovine adenovirus for its replication (Handa & Carter, 1979;Myers et al, 1980). A near-complete 4589 base sequence was assembled from 37 433 reads that shared 98 % nucleotide identity to the reference sequence (Gen-Bank AY388617).…”

Section: Bovine Adeno-associated Virus (Baav)supporting

confidence: 82%

Metagenomic characterization of the virome associated with bovine respiratory disease in feedlot cattle identified novel viruses and suggests an etiologic role for influenza D virus

Mitra

Cernicchiaro

Torres³

et al. 2016

Journal of General Virology

155

199

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Bovine respiratory disease (BRD) is the most costly disease affecting the cattle industry. The pathogenesis of BRD is complex and includes contributions from microbial pathogens as well as host, environmental and animal management factors. In this study, we utilized viral metagenomic sequencing to explore the virome of nasal swab samples obtained from feedlot cattle with acute BRD and asymptomatic pen-mates at six and four feedlots in Mexico and the USA, respectively, in April-October 2015. Twenty-one viruses were detected, with bovine rhinitis A (52.7 %) and B (23.7 %) virus, and bovine coronavirus (24.7 %) being the most commonly identified. The emerging influenza D virus (IDV) tended to be significantly associated (P=0.134; odds ratio=2.94) with disease, whereas viruses commonly associated with BRD such as bovine viral diarrhea virus, bovine herpesvirus 1, bovine respiratory syncytial virus and bovine parainfluenza 3 virus were detected less frequently. The detection of IDV was further confirmed using a real-time PCR assay. Nasal swabs from symptomatic animals had significantly more IDV RNA than those collected from healthy animals (P=0.04). In addition to known viruses, new genotypes of bovine rhinitis B virus and enterovirus E were identified and a newly proposed species of bocaparvovirus, Ungulate bocaparvovirus 6, was characterized. Ungulate tetraparvovirus 1 was also detected for the first time in North America to our knowledge. These results illustrate the Selected complete or partial genome sequences were submitted to GenBank/EMBL/DDBJ and accession numbers were obtained: BRBV strain

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Section: Bovine Adeno-associated Virus (Baav)supporting

confidence: 82%

Metagenomic characterization of the virome associated with bovine respiratory disease in feedlot cattle identified novel viruses and suggests an etiologic role for influenza D virus

Mitra

Cernicchiaro

Torres³

et al. 2016

Journal of General Virology

155

199

View full text Add to dashboard Cite

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“…Stocks of the Ad5 host-range mutants hr6 and hrl3 (provided by J. Williams), and sub316 (provided by T. Shenk) were grown in 293 cells. Stocks of the temperature-sensitive mutants AdSts215 (provided by W. Doerfler) and AdStsl49 (provided by H. Ginsberg) were prepared in KB cells as described previously (39). Herpes simplex virus types 1 and 2 (HSV-1 and HSV-2) were provided by I. Hay, and new stocks were grown on Vero cells.…”

Section: Methodsmentioning

confidence: 99%

Latent infection of KB cells with adeno-associated virus type 2

Laughlin¹,

Cardellichio²,

Coon³

1986

J Virol

Self Cite

102

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Adeno-associated virus (AAV) is a prevalent human virus whose replication requires factors provided by a coinfecting helper virus. AAV can establish latent infections in vitro by integration of the AAV genome into cellular DNA. To study the process of integration as well as the rescue of AAV replication in latently infected cells after superinfection with a helper virus, we established a panel of independently derived latently infected cell clones. KB cells were infected with a high multiplicity of AAV in the absence of helper virus, cloned, and passaged to dilute out input AAV genomes. AAV DNA replication and protein synthesis were rescued from more than 10% of the KB cell clones after superinfection with adenovirus type 5 (Ad5) or herpes simplex virus types 1 or 2. In the absence of helper virus, there was no detectable expression of AAV-specific RNA or proteins in the latently infected cell clones. AdS superinfection also resulted in the production of infectious AAV in most cases. All mutant adenoviruses tested that were able to help AAV DNA replication in a coinfection were also able to rescue AAV from the latently infected cells, although one mutant, Ad5hr6, was less efficient at AAV rescue. Analysis of high-molecular-weight cellular DNA indicated that AAV sequences were integrated into the cell genome. The restriction enzyme digestion patterns of the cellular DNA were consistent with colinear integration of the AAV genome, with the viral termini present at the cell-virus junction. In addition, many of the cell lines appeared to contain head-to-tail concatemers of the AAV genome. The understanding of the integration of AAV DNA is increasingly important since AAV-based vectors have many advantages for gene transduction in vitro and in vivo.

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“…In addition, Ostrove and Berns [66] reported that E1B is required for rescue of AAV DNA from the integrated state in human Detroit 6 cells but not for DNA replication. For the latter process, early region 4 appears to be necessary [67,68], and region E2A and VAI RNA are said to be involved in post-transcriptional regulation of capsid protein synthesis [69]. The requirement for VAI RNA during replication of AAV does not seem to be absolute.…”

Section: Helper Functions and The Replication Of Dependovirusesmentioning

confidence: 99%

Parvoviruses: agents of distinct pathogenic and molecular potential

Siegl

Tratschin

1987

FEMS Microbiology Letters

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Parvoviruses are small, spherical and extremely stable particles with a genome of linear single‐stranded DNA. They are widespread in nature and thus have been in virtually every animal species and in man. Many parvoviruses were initially isolated by chance and could not be associated with clinical disease. Today, however, a considerable number of these agents are known to cause a broad spectrum of syndromes including fetal death, malformations, dwarfism, cerebellar hypolasia and ataxia, enteritis, panleukopenia, aplastic anemia, and immunologic disorders. Biological, pathological, and molecular studies revealed that all these syndromes are explainable on the basis of two predominant requirements for productive cytolytic parvovirus replication: the availability of cellular helper function(s) provided exclusively by mitotically active cells in the phase of cellular DNA replication, and by apparently specific, yet so far undefined cellular functions

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Adenovirus helper function for growth of adeno-associated virus: effect of temperature-sensitive mutations in adenovirus early gene region 2

Cited by 69 publications

References 52 publications

Metagenomic characterization of the virome associated with bovine respiratory disease in feedlot cattle identified novel viruses and suggests an etiologic role for influenza D virus

Metagenomic characterization of the virome associated with bovine respiratory disease in feedlot cattle identified novel viruses and suggests an etiologic role for influenza D virus

Latent infection of KB cells with adeno-associated virus type 2

Parvoviruses: agents of distinct pathogenic and molecular potential

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