The modified cationic silica based stimuli responsive polymer–clay nanocomposite with pH responsive release manner could improve the targeting performance.
Here a novel antibacterial nanocomposite was developed for combination cancer therapy. The synthesized nanocarrier was characterized by FTIR, H NMR, thermogravimetric analysis (TGA), and FESEM-EDX. Its antibacterial activity was assessed by determining minimum inhibitory concentration (MIC) values. Doxorubicin (DOX) and methotrexate (MTX) conjugation with nanocarrier sustained the release of both drugs with apparent pH-triggered manner. Co-administration of DOX with MTX leads to an efficient anticancer performance to MCF7 cell lines verified by qRT-PCR and MTT assay tests. It was concluded that this novel drug delivery vehicle makes antibacterial and anticancer therapeutic processes proceed spontaneously, representing more efficient drug delivery system in nanomedicine. [Formula: see text].
The aim of this study is to isolate and identify Lactobacillus plantarum isolates from traditional cheese, Kouzeh, and evaluate their antimicrobial activity against some food pathogens. In total, 56 lactic acid bacteria were isolated by morphological and biochemical methods, 12 of which were identified as Lactobacillus plantarum by biochemical method and 11 were confirmed by molecular method. For analyzing the antimicrobial activity of these isolates properly, diffusion method was performed. The isolates were identified by 318 bp band dedicated for L. plantarum. The isolated L. plantarum represented an inhibitory activity against four of the pathogenic bacteria and showed different inhibition halos against each other. The larger halos were observed against Staphylococcus aureus and Staphylococcus epidermidis (15 ± 0.3 and 14.8 ± 0.7 mm, respectively). The inhibition halo of Escherichia coli was smaller than that of other pathogen and some L. plantarum did not show any inhibitory activity against E. coli, which were resistant to antimicrobial compounds produced by L. plantarum. The isolated L. plantarum isolates with the antimicrobial activity in this study had strong probiotic properties. These results indicated the nutritional value of Kouzeh cheese and usage of the isolated isolates as probiotic strains.
Aims: In order to improve survival in gastrointestinal conditions, probiotic bacteria, i.e. we developed a new encapsulation method of probiotics based on zeolite and by calcium-alginate zeolite-starch as an effective method for safer delivery of probiotics. Material and Methods: Lactobacillus casei (ATCC 39392) and Lactobacillus plantarum (ATCC 29521) were used as probiotics. After microorganism’s immobilization on zeolite base, encapsulation was done by calcium-alginate zeolite-starch with the extrusion method. Afterwards, they were incubated in the simulated gastric and intestinal condition. Viability of these bacteria was calculated on the basis of time required to reduce the logarithmic base in the microbial population. SEM techniques were used to study the appearance of the capsules. Results: after incubation for 120 min under simulated gastric condition and 180 min in intestinal juice conditions (pH=8.2), the count of alive L. casei and L. plantarum cells were 6.3 log cfu/ml and 7.3 log cfu/ml. However, for controlling the cells, this value was zero and 2.1 log cfu/ml respectively. Conclusion: According to the results, we had an increase of the survival rate of L. casei and L. plantarum in gastroesophageal-intestine simulated condition. These results indicated good efficacy and high performance of zeolite for immobilization and microencapsulation of probiotics.
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