In this study, determination of the amino acid profile of bee pollen produced in different parts of Turkey and performed to determine the protein level. The research was carried out with a total of 90 specimens from East-Southeast Anatolia, Central Anatolia, Black Sea, Marmara, Aegean and Mediterranean. According to the analysis results, alanine (143,40 ± 0,0) in bee pollen on mg / g basis, arginine (34,45 ± 0.0), histidine (32,55 ± 0.09), isoleucine (20,94 ± 1,24), leucine (23, 75 ± 1.27), lysine (31.15 ± 0.43), methionine (39.10 ± 1.32), phenylalanine (20.09 ± 0.95), proline (309.05 ± 28.56) and valine (15.79 ± 0.88) amino acids were detected. According to the analysis results, protein level of bee pollen in mg / g basis in Black Sea region (127,27 ± 0.31), Marmara region (117,56 ± 0.31), Mediterranean region (115,66 ± 0.31), Central Anatolia (115,09 ± 0.31), Aegean region (110,06 ± 0.31) and East-South East Anatolia (124,9 ± 0.31). The differences between the regions were found in Protein and amino acid levels (P