The development and expansion of road networks have profoundly impacted the natural landscape and various life forms. Animals are affected by these roads in a myriad of ways, none as devastating as road mortalities. This article reviews the literature on the magnitude, spatiotemporal patterns, factors, and consequences of Animal-Vehicle Collisions (AVCs) and the subsequent road kills. Furthermore, the review paper briefly outlines the relationship between roads and animals in the surrounding landscape and later examines the nature and impacts of AVCs. This article evaluates the statistics on the number of road kills and a critical analysis of the spatiotemporal patterns of these mortalities is also evaluated. Subsequently, the review paper examines current mitigation measures and the challenges impeding their success. The paper then concludes with an evaluation of geospatial tools (GIS) and other technologies used in road kill studies. The relevant findings of this paper are that,(1) factors influencing road kill patterns interact with one another; (2) AVCs have serious environmental, economic and social consequences; (3) road kill mitigation strategies suffer several challenges hindering their success; and (4) specific geospatial tools and other technologies have been utilised in assessing AVC road kill patterns. The review, therefore, recommends including overall road kill clusters of all animals in mortality surveys, increasing the spatial coverage of road kill observations, consistent surveying, sufficient research on nighttime driving distances and speed, utilising citizen science in all road mortality studies and incorporating GIS into all apps used for recording road kills. An increased sufficiency in road kill data coupled with improved technologies can enable more effective mitigation strategies to prevent AVCs.