Serological differentiations of physiological stages of Deoía fiavopicta (Stal,1854) (Homoptera:Cercopidae)The physiological stages of Daoia fiaroaicta (Stal, 1854) were differentiated by serology technique. Specific antiserum for newly laid eggs (AS-R) was used iri reactions with antigens obtained for each of the stages of the insect. The reactions showed that the egg stage displayed a maximurn of four lines: a, b, c and d. Nyrnphs displayed a rnaximum of two precipitation lines: lines b and d. For the adult stage, the males dis played a maximum of three lines: lines a, b and y and fernales displayed a maximum of eight precipitation lines: lines a, b, c, d, e, f, g and y.
Utilization of Deois flavopicta eggs (Stal, 1854) (Homoptera: Cercopidae) for the preparation of the specific antiserum This research dimed to develop and test serologyonDeois flavopicta (Stal, 1854).For the preparation of the specific antiserum, rabbits were immunized by the "lyrnphonodule" injection method with antigens obtained from D. fiavopicta eggs under the foliowing conditions: newly oviposited or up to two days old eggs; diapausing eggs, older than 30 days; and eggs with cap or with a mean of 15 days. Specific serogical reactions were obtained afterthe first antigen inoculation.The antiserum for newly laid eggs (AS-R) was more sensibie than antiserum for diapausing eggs (AS-D) and this was more sensible than antiserum for eggs with cap (AS-O).
RESUMOO presente trabalho teve como objetivo utilizar ovos de Deois fiavopicta (Stal, 1854) no preparo de antissoros específicos.Recebido em 03/1 1/87 1 Trabalho realizado com financiamento parcial da EMBRAPA.
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