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Histones are the major eukaryotic DNA-binding proteins. Posttranslational modifications on N-terminal tails of histones that form nucleosomes are often associated with distinct biological functions. Some theories suggest that one of these modifications, the phosphorylation of histone H3 at serine 10 (H3S10ph) plays a role in both chromosome condensation and sister chromatid cohesion. Although histones and some of their modifications are highly conserved, studies have shown that role and distribution of H3S10ph may differ between species. We evaluated the pattern of H3 phosphorylation using immunodetection during mitosis and meiosis in both diploid and tetraploid genotypes of Brachiaria species. Results revealed differences in chromosome distribution of H3S10ph when mitosis and meiosis were compared. Whole chromosomes were phosphorylated during meiosis I, whereas phosphorylation was restricted to the pericentromeric region in both meiosis II and mitosis. There was no variation in phosphorylation patterns between Brachiaria species and diploid and tetraploid genotypes. Regarding spatiotemporal coordination in the Brachiaria species evaluated, H3S10ph is related to maintenance of sister chromatid cohesion during cell divisions.

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This study analyzed the spatial distribution of Yellow Sigatoka Leaf Spot relative to soil fertility and plant nutritional status using geostatistics. The experimental area comprised 1.2 ha, where 27 points were georeferenced and spaced on a regular grid 18 9 18 m. The severity of Yellow Sigatoka, soil fertility and plant nutritional status were evaluated at each point. The spherical model was adjusted for all variables using restricted maximum likelihood. Kriging maps showed the highest infection rate of Sigatoka occurred in high areas of the field which had the highest concentration of sand, while the lowest disease was found in lower areas with lower silt, organic matter, total exchangeable bases, effective cation exchange capacity, base saturation, Ca and Mg in soil, and foliar sulfur (S). These results may help farmers manage Yellow Sigatoka disease more effectively, with balanced fertilization and reduced fungicide application. This practice minimizes the environmental impact and cost of production while contributing to production sustainability.

A nutrição equilibrada pode auxiliar no controle de doenças do cafeeiro. Assim, objetivou-se avaliar o efeito da adubação nitrogenada e potássica na intensidade da mancha aureolada do cafeeiro, em câmara de crescimento. Utilizou-se a cultivar Mundo Novo 376/4, suscetível à doença. Os tratamentos consistiram de cinco doses de N (3, 7, 11, 15 e 19 mmol L-1) combinadas com cinco doses de K (3, 5, 7, 9 e 11 mmol L-1), em esquema fatorial 5 x 5, totalizando 25 tratamentos e três repetições compostas por duas mudas em solução nutritiva. O delineamento experimental foi o inteiramente casualizado. Avaliou-se a incidência e a severidade da doença. A área abaixo da curva de progresso da incidência (AACPDI) e da severidade (AACPDS) foi calculada. Observou-se a maior incidência da doença (97,2%) na combinação das maiores doses de N e K. Houve diferença significativa apenas para o N na AACPDS, massa da planta seca e clorofilas a, b e total. A menor AACPDS (7,79) foi obtida com a dose 13,34 mmol L-1 de N. Houve correlação significativa e positiva para as clorofilas a, b e total com o N e a AACPS.

Studies on morphological plasticity are extremely important to plant breeding seeking to optimize genotype interactions with their environments. The present study evaluated 38 genotypes of Brachiaria ruziziensis cultivated during the rainy (October to March) and dry (April to September) seasons and examined variations in their anatomical characteristics. Fully expanded leaves were collected and fixed in FAA 70 (formaldehyde: acetic acid: ethyl alcohol) for 72h and subsequently stored in 70% alcohol. Median sections of leaf blades were examined and evaluated according to traditional plant anatomical procedures. Genotypes 15, 27, 40, 53, and 90 demonstrated interactions with the environment in five of the anatomical characteristics evaluated; genotypes 1 and 33 presented no plasticity in any of the characteristics examined. Except for the thickness of abaxial (ABT) and adaxial (ADT) epidermal surfaces, the genotypes showed different behaviors for all the other characteristics evaluated: mesophyll thickness (MT), xylem diameter (XD), distance between bundles (DB), number of bundles (NB), number of bulliform cells (NBC), sclerenchyma tissue area (%L), and bulliform cell area (ABC).

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