1. The dependence of force (F) on length (L) in smooth muscle remains uncertain since (i) it is influenced by changes in activation (myosin light chain phosphorylation), (ii) no anatomical reference length for the contractile unit is available, (iii) the length at which optimum force is generated (Lo) exhibits a broad, flat optimum, and (iv) the presence of an extensive connective tissue network makes it difficult to stretch tissues without damage. 2. A swine carotid medial ring preparation prepared by removal of the adventitia and endothelium could be stretched to 1P8 Lo without decreasing active force generation on return to shorter lengths. 3. A highly reproducible mechanically defined reference length, Lo, was obtained by fitting force-length data between 0 3 and 1P6 Lo with a third-order polynomial where L =Lo when dF/dL = 0. 4. Activation as assessed by myosin regulatory light chain (MRLC) phosphorylation increased with length in 100 /AM histamine-stimulated tissues from 0 6 to 1I8 Lo. 5. Activation was constant in K+-depolarized and field-stimulated tissues from 1 0 to 1.8 Lo allowing determination of the descending limb of the force-length relation to be assessed independently of activation. 6. The slope of the descending limb of the force-length relation was linear except at very long lengths, which often produced tissue damage. The slope was not statistically different from that estimated for sarcomeres in vertebrate skeletal muscle. 7. The medial ring preparation and the procedures used to define the reference length provide advantages for the measurement of length-dependent variables.
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